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Displaying 301 - 325 of 484

Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome

October 17, 2003
Author(s)
John M. Butler, R Schoske, Peter Vallone
A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390

Genomic DNA of Nostoc Commune (Cyanobacteria) Becomes Covalently Modified During Long-Term (Decades) Desiccation But Is Protected From Oxidative Damage and Degradation

June 15, 2003
Author(s)
B Shirkey, N J. McMaster, Sue C. Smith, D J. Wright, H Rodriguez, Pawel Jaruga, M Birincioglu, R F. Helm, M Potts
Genomic DNA of Nostoc commume (Cyanobacteria) became covalently modified during decades of desiccation. Amplification of gene loci from desiccated cells required pretreatment of DNA with N-phenacylthiazolium bromide; a reagent that cleaves DNA- and protein

Specific Antibody-DNA Interaction: A Novel Strategy for Tight DNA Recognition

May 27, 2003
Author(s)
S M. Di Pietro, J M. Centeno, M L. Cerutti, M F. Lodeiro, D U. Ferreiro, L G. Alonso, Frederick P. Schwarz, F Goldbaum, G de Prat-Gay
Taking advantage of the extreme stability of a protein-DNA complex, we obtained highly specific monoclonal antibodies against a predetermined palindromic double stranded DNA sequence, corresponding to the binding site of the E2 transcriptional regulator of

Arabidopsis Thaliana Ogg1 Protein Excises 8-Hydroxyguanine and 2,6- Diamino-4-hydroxy-5-formamidopyrimidine from Oxidative Damaged DNA Containing Multiple Lesions

March 18, 2003
Author(s)
T Morales-Ruiz, M Birincioglu, Pawel Jaruga, H Rodriguez, T. Roldan-Arjona, Miral M. Dizdar
A functional homologue of eukaryotic Ogg1 proteins in the model plant Arabidopsis thaliana has recently been cloned, isolated, and characterized [Garcia-Ortiz, M.V., Ariza, R.R., and Roldan-Arjona, T. (2001) Plant Mol. Biol. 47, 795-804]. This enzyme

DNA-Based Sensors

January 1, 2003
Author(s)
Michael J. Tarlov, A B. Steel
The development of DNA-based sensors for genetic assay and sequencing applications has exploded over the last decade. Nearly all of these devices rely on the hybridization of a complementary oligonucleotide with surface-confined, single-stranded DNA or RNA
Displaying 301 - 325 of 484
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