U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Search Publications

NIST Authors in Bold

Displaying 101 - 125 of 241

Concordance Study Between the AmpFlSTR((R)) MiniFiler(TM) PCR Amplification Kit and Conventional STR Typing Kits

July 25, 2007
Author(s)
Carolyn R. Steffen, Margaret C. Kline, Julio J. Mulero, Robert E. Lagace, Chien-Wei Chang, Lori K. Hennessy, John M. Butler
The AmpFlSTR MiniFiler PCR Amplification kit developed by Applied Biosystems enables size reduction on eight of the larger short tandem repeat (STR) loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded DNA

Analysis of artificially degraded DNA using STRs and SNPs results of a collaborative European (EDNAP) exercise

December 1, 2006
Author(s)
L. A. Dixon, A. E. Dobbins, H. Pulker, John M. Butler, Peter M. Vallone, Michael D. Coble, W. Parson, B. Berger, P. Grubwieser, H. S. Mogensen, N. Morling, K. Nielsen, J. J. Sanchez, E. Petkovski, A. Carracedo, P. Sanchez-Diz, E. Ramos-Luis, M. Brion, J. A. Irwin, R. S. Just, O. Loreille, T. J. Parsons, D. Syndercombe-Court, H. Schmitter, B. Stradmann-Bellinghausen, K. Bender, P. Gill

Analysis of potential cancer biomarkers in mitochondrial DNA

December 1, 2006
Author(s)
J P. Jakupciak, G. D. Dakubo, Samantha D. Maragh, R. L. Parr
Understanding mitochondrial biology is a fundamental research goal in human genetics and medicine. The use of mitochondria to serve as a biomarker is rapidly expanding in disciplines ranging from cancer, rare metabolic diseases, aging, the tracing of human

An Optimized Electrophoresis System for Tandem SSCP and Heteroduplex Analysis of p53 Gene Exons 5-9 on Glass Microfluidic Chips

October 1, 2006
Author(s)
Christa N. Hestekin, J P. Jakupciak, Thomas N. Chiesl, C D. O'Connell, Annelise E. Barron, C W. Kan
With the sequencing of the human genome, there is a growing need for rapid and sensitive genotyping methods that can be incorporated into the clinical setting. DNA-based methods, such as single strand conformational polymorphism (SSCP) and heteroduplex

Standards in Gene Expression Microarray Experiments

August 1, 2006
Author(s)
Marc L. Salit
The use of standards in gene expression measurements with DNA microarrays is ubiquitous—they just are not yet the kind of standards that have yielded microarray gene expression profiles that can be readily compared across different studies and different

The Pseudo-mitochondrial Genome Influences Mistakes in Heteroplasmy Interpretation

July 21, 2006
Author(s)
Ryan L. Parr, Jennifer Maki, Brian Reguly, Gabriel D. Dakubo, A A. Aguirre, Roy Wittock, Kerry Robinson, J P. Jakupciak, Robert E. Thayer
Nuclear mitochondrial pseudogenes (numts) are a potential source of contamination during mitochondrial DNA PCR amplification. This paper is the first to fully sequence 29 paralogous nuclear DNA fragments that represent the entire mitochondrial genome. This

Structural alterations in breast stromal and epithelial DNA: the influence of 8,5'-cyclo-2'-deoxyadenosine

June 1, 2006
Author(s)
K. M. Anderson, Pawel Jaruga, C. R. Ramsey, N. K. Gilman, V. M. Green, S. W. Rostad, J. T. Emerman, M Miral Dizdar, D. C. Malins
(5'S)-8,5'-Cyclo-2'-deoxyadenosine (S-cdA), which arises from the reaction of the hydroxyl radical (*OH) with 2'-deoxyadenosine in DNA, is a lesion comprising a base-sugar linkage that distorts the DNA backbone. This structure impedes transcription and

Setting standards and developing technology to aid the human identity testing community

April 1, 2006
Author(s)
John M. Butler, Michael D. Coble, Amy E. Decker, David L. Duewer, Carolyn R. Steffen, Margaret C. Kline, Janette W. Redman, Peter Vallone
Our project team at the U.S. National Institute of Standards and Technology (NIST) is funded by the National Institute of Justice (NIJ) to conduct research that benefits the human identity testing community and to create tools that enable forensic DNA

The evaluation of an autosomal SNP 12-plex assay

April 1, 2006
Author(s)
Peter M. Vallone, Amy E. Decker, Michael D. Coble, John M. Butler
SNPs have the potential to play a useful role in human identification testing. Small PCR amplicon sizes associated with SNP typing technologies make SNPs attractive for typing degraded DNA or other low copy number situations. SNP markers can be useful in

Analysis of DNA Single Nucleotide Polymorphisms by Mass Spectrometry

March 17, 2006
Author(s)
Peter Vallone, John Butler
Single nucleotide polymorphisms(SNP) are the most frequent form of DNA sequence variation in the human genome and are becoming increasingly useful as genetic markers for genome mapping studies, medical diagnostics, and human identity testing. The primer

Oxidative Stress and DNA Damage-DNA Repair System in Vascular Smooth Muscle Cells in Artery and Vein Grafts

March 1, 2006
Author(s)
S. H. McLaren, D. Gao, L. Chen, J. R. Eshleman, V. Dawson, M. A. Trush, V. Bohr, M. Dizdaroglu, G. M. Williams, C. Wei
Graft failure in coronary artery bypass grafts (CABGs) utilizing the saphenous vein is significantly higher than in those utilizing the internal mammary artery (IMA) or the radial artery (RA). While a number of studies have described this phenomenon
Displaying 101 - 125 of 241