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Displaying 1 - 25 of 228

"The devil's in the detail": Release of an expanded, enhanced and dynamically revised forensic STR Sequence Guide

May 13, 2021
Author(s)
Christopher Phillips, Katherine Gettings, Jonathan King, David Ballard, Martin Bodner, Lisa Borsuk, Walther Parson
The STR sequence template file published in 2016 as part of the considerations from the DNA Commission of theInternational Society for Forensic Genetics on minimal STR sequence nomenclature requirements, has been comprehensively revised and audited using

Classification of STR allelic variation using massively parallel sequencing and assessment of flanking region power

May 12, 2021
Author(s)
Laurence Devesse, Lucinda Davenport, Lisa Borsuk, Katherine Gettings, Gabriella Mason-Buck, Peter Vallone, David Ballard
The application of massively parallel sequencing (MPS) to forensic genetics has led to improvements in multiple aspects of DNA analysis, however, additional complexities are concurrently associated with these advances. In relation to short tandem repeat

Revision of the SNPforID 34-plex forensic ancestry test: Assay enhancements, standard reference sample genotypes and extended population studies

May 3, 2021
Author(s)
John M. Butler, Manuel Fondevila, Carla Santos, Ana Freire, Christopher Phillips, M Lareu, Peter Vallone
A revision of an established 34 SNP forensic ancestry test has been made by swapping the under- performing rs727811 component SNP with the highly informative rs3827760 that shows a near-fixed East Asian specific allele. We collated SNP variability data for

Sequence-based U.S. population data for 7 X STR loci

April 26, 2021
Author(s)
Lisa Borsuk, Carolyn R. Steffen, Kevin M. Kiesler, Peter Vallone, Katherine Gettings
The National Institute of Standards and Technology (NIST) U.S. population sample set of unrelated individuals was used to determine allele and haplotype frequencies for seven X-chromosome short tandem repeat (STR) loci in four linkage groups. DXS7132

Results of the 2018 Rapid DNA Maturity Assessment

January 27, 2020
Author(s)
Erica L. Romsos, Peter M. Vallone
Three commercially available integrated rapid DNA instruments were tested as a part of a rapid DNA maturity assessment in the July of 2018. The assessment was conducted with sets of blinded single-source reference samples to gauge the typing success of the

Understanding the characteristics of sequence-based single-source DNA profiles

November 9, 2019
Author(s)
Sarah Riman, Hariharan K. Iyer, Lisa A. Borsuk, Peter M. Vallone
The sequencing of STR markers provides additional information present in the underlying sequence variation that is typically masked by traditional fragment-based genotyping. However, the interpretation of STR profiles generated by targeted sequencing

Report from the STRAND Working Group on the 2019 STR Sequence Nomenclature Meeting

September 21, 2019
Author(s)
Katherine Gettings, David Ballard, Martin Bodner, Lisa Borsuk, Jonathan King, Walther Parson, Christopher Phillips
This report summarizes topics discussed at the STR sequence nomenclature meeting hosted by the STRAND Working Group in April 2019. Invited attendees for this meeting included researchers known-to-us to be developing STR sequence-based nomenclature schemata

2018 Rapid DNA Maturity Assessment Results

September 26, 2018
Author(s)
Erica L. Romsos, Peter M. Vallone
The Rapid DNA Act, which amends the DNA Identification Act of 1994, allows for the integration of rapid DNA instruments for use by law enforcement for DNA testing of arrestees in a booking station environment. Several parallel efforts have been made to

Sequence-based U.S. population data for 27 autosomal STR loci

July 19, 2018
Author(s)
Katherine B. Gettings, Lisa A. Borsuk, Carolyn R. Steffen, Kevin M. Kiesler, Peter M. Vallone
This manuscript reports STR sequence-based allele frequencies for the "NIST 1036" sample set across 27 autosomal STR loci: D1S1656, TPOX, D2S441, D2S1338, D3S1358, D4S2408, FGA, D5S818, CSF1PO, D6S1043, D7S820, D8S1179, D9S1122, D10S1248, TH01, vWA

Programmable assembly of three-dimensional binary superlattices from multi-flavored DNA- functionalized particles

July 10, 2018
Author(s)
Evan Pretti, Hasan Zerze, Minseok Song, Yajun Ding, Nathan Mahynski, Harold W. Hatch, Vincent K. Shen, Jeetain Mittal
Programmable self-assembly of nano- or micron-sized colloidal particles can be achieved by grafting single- stranded DNA sequences onto the surfaces of colloids. However, this assembly is traditionally premised on the pairwise interaction between a single

Sequence-based U.S. population data for the SE33 locus

May 7, 2018
Author(s)
Lisa Borsuk, Katherine Gettings, Carolyn R. Steffen, Kevin M. Kiesler, Peter Vallone
A set of 1036 U.S. Population Samples were sequenced using the Illumina ForenSeq DNA Signature Prep Kit. This sample set has been highly characterized using a variety of marker systems for human identification. The FASTQ files obtained from a ForenSeq DNA

STRSeq: A catalog of sequence diversity at human identification Short Tandem Repeat loci

November 1, 2017
Author(s)
Katherine Gettings, Lisa Borsuk, D Ballard, Martin Bodner, Bruce Budowle, Laurence Devesse, Jonathan King, W. Parson, Christopher Phillips, Peter Vallone
The STR Sequencing Project (STRSeq) was initiated to facilitate the description of sequence- based alleles at the Short Tandem Repeat (STR) loci targeted in human identification assays. This international collaborative effort, which has been endorsed by

DNA Commission of the International Society for Forensic Genetics: Recommendations on the validation of software programs performing biostatistical calculations for forensic genetics applications

September 4, 2016
Author(s)
Michael D. Coble, John S. Buckleton, John M. Butler, Barbara Guttman
The use of biostatistical software programs to assist in data interpretation and calculate likelihood ratios is essential to forensic geneticists and part of the daily case work flow for both kinship and DNA identification laboratories. Previous

Evaluation of Forensic DNA Mixture Evidence: Guidelines for Evaluation, Interpretation, and Statistical Calculations using the Combined Probability of Inclusion

August 31, 2016
Author(s)
Frederick R. Bieber, John Buckleton, Bruce Budowle, John M. Butler, Michael D. Coble
The evaluation and interpretation of forensic DNA mixture evidence faces greater interpretational challenges due to increasingly complex mixture evidence. Such challenges include: casework involving low quantity or degraded evidence leading to allele and

Is the Factor of 10 Still Applicable Today?

July 13, 2016
Author(s)
Simone N. Gittelson, John S. Buckleton
The assignment of the weight of DNA evidence depends on a number of factors (allele probability estimates, the population genetic model used, the value of the coancestry coefficient, etc.). One of these factors is the allele probability estimates from a

Expected net gain data of low-template DNA analyses

May 30, 2016
Author(s)
Simone N. Gittelson, Carolyn R. Steffen, Michael D. Coble
Low-template DNA analyses are affected by stochastic effects which can produce a configuration of peaks in the electropherogram (EPG) that is different from the genotype of the DNA's donor. A probabilistic and decision-theoretic model can quantify the

Low-template DNA: A single DNA analysis or two replicates?

April 18, 2016
Author(s)
Simone N. Gittelson, Carolyn R. Steffen, Michael D. Coble
This study investigates the following two questions: (i) Should the DNA analyst concentrate the DNA extract into a single amplification or should he/she split it up to do two replicates? (ii) Given the electropherogram obtained from a first analysis, is it

Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae

January 25, 2016
Author(s)
Sandra M. Da Silva, Lindsay Harris, Nathanael D. Olson, Steven P. Lund, Autumn S. Downey, Zvi Kelman, Marc L. Salit, Jayne D. Morrow
Aims: We describe the development and interlaboratory study of modified Saccharomyces cerevisiae as a candidate material to evaluate a full detection workflow including DNA extraction and quantitative polymerase chain reaction (qPCR). Methods and results
Displaying 1 - 25 of 228