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Health / Regenerative medicine and advanced therapy

Flow cytometry

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Displaying 1 - 15 of 15

Measuring the size of oil droplets in a flow cytometer using Mie Resonances

January 21, 2025
Author(s)
Richard E. Cavicchi, Dean Ripple, Joshua Welsh, Jerilyn Izac, Alexander Peterson, Aaron Goldfain, Wyatt Vreeland
An emulsion of silicone oil droplets in aqueous buffer produces a distinctive series of peaks or resonances in the side scatter histogram in a flow cytometer. As many as 12 peaks are observed in the violet-side scatter channel at 405 nm, with half that

Standardization of Flow Cytometric Detection of Antigen Expression

January 25, 2024
Author(s)
Linhua Tian, Aaron Nelson, Tyler Lowe, Linda Weaver, Constance Yuan, Paul DeRose, Maryalice Stetler-Stevenson, Lili Wang
Background: While response to antigen-based immunotherapy relies upon the level of antigen expression by tumor cells and as decreasing levels of antigen expression can be an early indicator of developing resistance to therapy, we have developed an antigen

Monoclonal Antibodies as SARS-CoV-2 Serology Standards: Experimental Validation and Broader Implications for Correlates of Protection

October 28, 2023
Author(s)
Lili Wang, Paul Patrone, Anthony Kearsley, Jerilyn Izac, Adolfas Gaigalas, John Prostko, Hang Xie, Linhua Tian, Elzafir Elsheikh, Edward Kwee, Troy Kemp, Simon Jochum, Natalie Thornburg, Clifford McDonald, Adi Gundlapalli, Sheng Lin-Gibson
COVID-19 has highlighted the need for more accurate and reproducible measurements of humoral immunity, including antibody levels and neutralization potential, which are critical for diagnostics, seroprevalence estimates, and development of vaccines and

Size and Number Concentration Measurements of Polystyrene Nanoparticle Suspensions using Several Different Techniques

September 8, 2022
Author(s)
Paul DeRose, Kurt D. Benkstein, Elzafir B. Elsheikh, Adolfas K. Gaigalas, Sean Lehman, Dean Ripple, Linhua Tian, Wyatt Vreeland, Adam York, Yu-Zhong Zhang, Hao-Wei Wang
The number concentrations of nominal 100 nm, 200 nm and 500 nm diameter, fluorescently-labeled polystyrene nanosphere suspensions were measured using seven different techniques. Diameter values were also measured where possible. The diameter values were

Measurement of serological and neutralizing antibodies specific to SARS-CoV-2 virus in humans with COVID-19 disease

March 8, 2021
Author(s)
Linhua Tian, Elzafir B. Elsheikh, Paul Patrone, Anthony Kearsley, adolfas Gaigalas, Sarah L. Inwood, Sheng Lin-Gibson, Dominic Esposito, Lili Wang
The global response to COVID-19 has led to rapid advances in diagnostic, surveillance, and vaccine development. Critical measurements underpinning many of these efforts include serological assays to assess seroprevalence of populations and the complex

Expanding NIST's Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles

September 15, 2020
Author(s)
Paul DeRose, Linhua Tian, Elzafir B. Elsheikh, Aaron Urbas, Yu-Zhong Zhang, Hao-Wei Wang
NIST, NIH and other industry stakeholders have been working together to enable fluorescence intensities of flow cytometer calibration beads to be assigned quantitative 'ERF' values with high accuracy and precision. The ultimate goal of this effort is to

An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes

September 2, 2020
Author(s)
Deepa Rajagopal, Linhua Tian, Shiqiu Xiong, Hao-Wei Wang, Jonathan Campbell, Luisa Saraiva, S Vessillier
Activation of T lymphocytes leads to differentiation, characterized by the ability to induce production of cytokines. Accurate determination of cellular subsets that secrete particular cytokine(s) is therefore, a significant parameter for functional

FLOW CYTOMETER PERFORMANCE CHARACTERIZATION, STANDARDIZATION AND CONTROL

May 8, 2017
Author(s)
Hao-Wei Wang, Robert A. Hoffman
Flow cytometry is a widely used technique for the analysis of single cells and particles. It is an essential tool for immunological research, drug and device development, clinical trials, disease diagnosis, and therapy monitoring. However, the measurements

Standardization, Calibration, and Control in Flow Cytometry

January 5, 2017
Author(s)
Hao-Wei Wang, Robert A. Hoffman
Standardization, control, and calibration provide different degrees of certainty about the data acquired with an instrument. Each process is aimed at assuring that results from the instrument have the quality required for the intended purpose. The purpose
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