An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes
Deepa Rajagopal, Linhua Tian, Shiqiu Xiong, Lili Wang, Jonathan Campbell, Luisa Saraiva, S Vessillier
Activation of T lymphocytes leads to differentiation, characterized by the ability to induce production of cytokines. Accurate determination of cellular subsets that secrete particular cytokine(s) is therefore, a significant parameter for functional characterization of an immunological response. The present study was conducted to develop a method to simultaneously measure intracellular cytokine positive CD4 and CD8 positive T cell numbers in a single tube with a no-wash protocol. We report here the development of a simplified, rapid procedure for accurate enumeration cytokine positive cells using BD Trucount tubes. This single step protocol allows for accurate enumeration and characterization of immune cell phenotype.
, Tian, L.
, Xiong, S.
, Wang, L.
, Campbell, J.
, Saraiva, L.
and Vessillier, S.
An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes, Journal of Immunology and Regenerative Medicine, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=929762
(Accessed September 21, 2023)