An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes

Published

September 2, 2020

Author(s)

Deepa Rajagopal, Linhua Tian, Shiqiu Xiong, Hao-Wei Wang, Jonathan Campbell, Luisa Saraiva, S Vessillier

Abstract

Activation of T lymphocytes leads to differentiation, characterized by the ability to induce production of cytokines. Accurate determination of cellular subsets that secrete particular cytokine(s) is therefore, a significant parameter for functional characterization of an immunological response. The present study was conducted to develop a method to simultaneously measure intracellular cytokine positive CD4 and CD8 positive T cell numbers in a single tube with a no-wash protocol. We report here the development of a simplified, rapid procedure for accurate enumeration cytokine positive cells using BD Trucount tubes. This single step protocol allows for accurate enumeration and characterization of immune cell phenotype.

Citation

Journal of Immunology and Regenerative Medicine

Pub Type

Journals

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Keywords

Cytokine positive T lymphocytes, enumeration, flow cytometry, single step protocol, two step protocol, Trucount bead standard, isotype control, INF-r

Citation

Rajagopal, D. , Tian, L. , Xiong, S. , Wang, H. , Campbell, J. , Saraiva, L. and Vessillier, S. (2020), An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes, Journal of Immunology and Regenerative Medicine, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=929762 (Accessed October 27, 2025)