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Size and Number Concentration Measurements of Polystyrene Nanoparticle Suspensions using Several Different Techniques

Published

Author(s)

Paul C. DeRose, Kurt D. Benkstein, Elzafir B. Elsheikh, Adolfas K. Gaigalas, Sean Lehman, Dean C. Ripple, Linhua Tian, Wyatt N. Vreeland, Adam York, Yu-Zhong Zhang, Lili Wang

Abstract

The number concentrations of nominal 100 nm, 200 nm and 500 nm diameter, fluorescently-labeled polystyrene nanosphere suspensions were measured using seven different techniques. Diameter values were also measured where possible. The diameter values were found to agree within 20%, but the number concentration values differed by as much as a factor of two. Accuracy and reproducibility related with the different techniques are discussed with the goal of establishing number concentration standards for sub-micrometer particles.
Citation
Nanomaterials
Volume
12

Keywords

bead, calibration, concentration, diameter, flow cytometry, nanosphere, NIST, number, reference materials, sub-micrometer, standards

Citation

DeRose, P. , Benkstein, K. , Elsheikh, E. , Gaigalas, A. , Lehman, S. , Ripple, D. , Tian, L. , Vreeland, W. , York, A. , Zhang, Y. and Wang, L. (2022), Size and Number Concentration Measurements of Polystyrene Nanoparticle Suspensions using Several Different Techniques, Nanomaterials, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=934905 (Accessed February 3, 2023)
Created September 8, 2022, Updated November 29, 2022