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This paper showcases recent research at NIST on quantitative hyperspectral imaging of single cells and tissues with a variety of imaging contrasts including scattering reflectance, transmission, and dark-field and with supervised and unsupervised data
The editors introduce a Journal of Biomedical Optics (JBO) feature issue on "Hyperspectral imaging." The special issue features a number of important research and review papers on new hyperspectral imaging and detection devices and associated technologies
Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of chemical
Jerilyn Izac, Edward Kwee, Linhua Tian, John Elliott, Lili Wang
COVID-19 is an ongoing, global pandemic caused by the novel, highly infectious SARS-CoV-2 virus. Efforts to dampen the effects of SARS-CoV-2, such as mass vaccination and development of monoclonal therapeutics, require precise measurements of correlative
Edward Kwee, Alexander Peterson, Michael Halter, John Elliott
Quantitative phase imaging (QPI) provides an approach for monitoring the growth rate of individual cells by measuring the optical pathlength of visible light as it is passes through cells. A distinct advantage of QPI is that the measurements are, in
Alexander Peterson, Alessandro Tona, Michael Halter, Anne Plant, John Elliott
Surface plasmon resonance microscopy (SPRM) is a powerful label-free imaging technique with spatial resolution approaching the optical diffraction limit. The high sensitivity of SPRM to small changes in index of refraction at an interface allows imaging of
Alexander Peterson, Michael Halter, Anne Plant, John Elliott
Surface plasmon resonance imaging (SPRI) allows real-time label-free imaging based on index of refraction, and changes in index of refraction at an interface. SPRI can be carried out on a microscope by launching light into a sample, and collecting
Alexander Peterson, Michael Halter, Alessandro Tona, Anne Plant
Background Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously shown that SPRI can be used to study the dynamics of cell-substratum interactions. However