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Bioscience

Bioimaging

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Displaying 1 - 7 of 7

Quantitative hyperspectral microscopy from single cell to tissue imaging

March 20, 2025

Author(s)

Jeeseong Hwang

This paper showcases recent research at NIST on quantitative hyperspectral imaging of single cells and tissues with a variety of imaging contrasts including scattering reflectance, transmission, and dark-field and with supervised and unsupervised data

JBO Special Issue on Hyperspectral Imaging

March 4, 2025

Author(s)

Baowei Fei, Jeeseong Hwang, Matija Milanic

The editors introduce a Journal of Biomedical Optics (JBO) feature issue on "Hyperspectral imaging." The special issue features a number of important research and review papers on new hyperspectral imaging and detection devices and associated technologies

Benchtop IR Imaging of Live Cells: Monitoring the Total Mass of Biomolecules in Single Cells

September 4, 2024

Author(s)

Yow-Ren Chang, Seongmin Kim, Young Jong Lee

Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of chemical

Practical application of microsphere samples for benchmarking a quantitative phase imaging system

December 19, 2020

Author(s)

Edward Kwee, Alexander Peterson, Michael Halter, John Elliott

Quantitative phase imaging (QPI) provides an approach for monitoring the growth rate of individual cells by measuring the optical pathlength of visible light as it is passes through cells. A distinct advantage of QPI is that the measurements are, in

Mass Measurements of Focal Adhesions in Single Cells Using High Resolution Surface Plasmon Resonance Microscopy

February 22, 2018

Author(s)

Alexander Peterson, Alessandro Tona, Michael Halter, Anne Plant, John Elliott

Surface plasmon resonance microscopy (SPRM) is a powerful label-free imaging technique with spatial resolution approaching the optical diffraction limit. The high sensitivity of SPRM to small changes in index of refraction at an interface allows imaging of

Surface plasmon resonance microscopy: achieving a quantitative optical response

September 7, 2016

Author(s)

Alexander Peterson, Michael Halter, Anne Plant, John Elliott

Surface plasmon resonance imaging (SPRI) allows real-time label-free imaging based on index of refraction, and changes in index of refraction at an interface. SPRI can be carried out on a microscope by launching light into a sample, and collecting

High Resolution Surface Plasmon Resonance Imaging for Single Cells

November 30, 2014

Author(s)

Alexander Peterson, Michael Halter, Alessandro Tona, Anne Plant

Background Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously shown that SPRI can be used to study the dynamics of cell-substratum interactions. However