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Standards

Reference materials

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Displaying 76 - 100 of 546

Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification

June 1, 2022
Author(s)
Jason Kralj, Dieter Tourlousse, Monique Hunter, Erica Romsos, Blaza Toman, Peter Vallone, Scott Jackson
Reference Material (RM) 8376 is intended for NGS-based measurements quantitative to the chromosome. A unit of RM 8376 consists of 20 components (A-T, 19 bacteria and 1 human) each containing well-characterized DNA in 10 mmol/L Tris-HCl, 1 mmol/L EDTA pH 8

Benchmarking challenging small variants with linked and long reads

May 11, 2022
Author(s)
Justin Wagner, Nathanael Olson, Lindsay Harris, Marc L. Salit, Fritz Sedlazeck, Chunlin Xiao, Justin Zook
Genome in a Bottle benchmarks are widely used to help validate clinical sequencing pipelines and develop variant calling and sequencing methods. Here we use accurate linked and long reads to expand benchmarks in 7 samples to include difficult-to-map

Certification of Standard Reference Material(R) 3655 Glycans in Solution (Frozen)

April 12, 2022
Author(s)
Mark Lowenthal, Blaza Toman, Brian Lang, Karen W. Phinney
Standard Reference Material (SRM) 3655 is intended primarily for use as a calibration standard for the measurement of enzymatically released N-linked glycans. Applications of SRM 3655 include the benchmarking and comparability of analytical techniques, as

Reference Materials for MS-based Untargeted Metabolomics and Lipidomics: A Review by the Metabolomics Quality Assurance and Quality Control Consortium (mQACC)

April 9, 2022
Author(s)
Katrice Lippa, William Davis, Christina Jones, Tracey Johnston, Juan Aristizabal-Henao, Richard Beger, John Bowden, Corey Broeckling, Chris Beecher, Warwick Dunn, Roberto Flores, Goncalo Gouveia, Thomas Hartung, Matthew Lewis, Ioanna Ntai, Andrew Percy, Dan Raftery, Jinchun Sun, Georgios Theodoridis, Fariba Tayyari, Federico Torta, Candice Ulmer, Baljit Ubhi
Introduction The metabolomics quality assurance and quality control consortium (mQACC) is enabling the identification, development, prioritization and promotion of suitable reference materials (RMs) to be used in quality assurance (QA) and quality control

A pyroelectric detector-based method for low uncertainty spectral irradiance and radiance responsivity calibrations in the infrared using tunable lasers

April 4, 2022
Author(s)
Brian Alberding, John Woodward, Ping-Shine Shaw, Leonard Hanssen, Catherine Cooksey, Joseph P. Rice
The standard uncertainty of detector-based radiance and irradiance responsivity calibrations in the short-wave infrared (SWIR) traditionally has been limited to around 1 % or higher by the low spatial uniformity of detectors used to transfer the scale from

A complete reference genome improves analysis of human genetic variation

April 1, 2022
Author(s)
Sergey Aganezov, Stephanie Yan, Daniela Soto, Melanie Kirsche, Samantha Zarate, Justin Wagner, Jennifer McDaniel, Nathanael David Olson, Rajiv McCoy, Megan Dennis, Justin Zook, Michael Schatz
Compared to its predecessors, the Telomere-to-Telomere CHM13 genome adds nearly 200 million base pairs of sequence, corrects thousands of structural errors, and unlocks the most complex regions of the human genome for clinical and functional study. We show

Models for an Ultraviolet-C Research and Development Consortium

March 25, 2022
Author(s)
Dianne L. Poster, C Cameron Miller, Yaw S. Obeng, John J. Kasianowicz, Michael T. Postek, Norman Horn, Troy Cowan, Richard Martinello
The development of an international, precompetitive, collaborative, ultraviolet (UV) research consortium is discussed as an opportunity to lay the groundwork for a new UV commercial industry and the supply chain to support this industry. History has

Certification of Standard Reference Material 3289 Multivitamin Tablets

March 18, 2022
Author(s)
Hugh Hayes, Jerome Mulloor, Michael Nelson, Kate Rimmer, Lee L. Yu, James H. Yen
Standard Reference Material® (SRM®) 3289 Multivitamin Tablets was developed as part of a collaborative effort between the National Institute for Standards and Technology (NIST) and the National Institutes of Health Office of Dietary Supplements (NIH ODS)

Certification of NIST Gas Mixture Standard Reference Materials

February 10, 2022
Author(s)
Christina Cecelski, Kimberly Harris, Cassie Goodman, William A. Kimes, Philip Liu, Walter R. Miller Jr., Jennifer Carney
The Gas Sensing Metrology Group at NIST provides accurate, stable gaseous standards for use in various types of emissions testing and environmental monitoring. The group develops and maintains the highest level of primary gas standards, which provide

Performance of NIST SRM(R) 2917 with 13 Recreational Water Quality Monitoring qPCR Assays

February 10, 2022
Author(s)
Jessica Willis, Mano Sivaganesan, Richard Haugland, Jason Kralj, Stephanie Servetas, Monique Hunter, Scott Jackson, Orin Shanks
Fecal pollution remains a significant challenge for recreational water quality management worldwide. In response, there is a growing interest in the use of real-time quantitative PCR (qPCR) methods to achieve same-day notification of recreational water

Curated variation benchmarks for challenging medically relevant autosomal genes

February 7, 2022
Author(s)
Justin Wagner, Nathanael David Olson, Lindsay Harris, Jennifer McDaniel, Fritz Sedlazeck, Chen-Shan Chin, Justin Zook
The repetitive nature and complexity of some medically relevant genes poses a challenge for their accurate analysis in a clinical setting. The Genome in a Bottle Consortium has provided variant benchmark sets, but these exclude nearly 400 medically

New reference materials for trace-levels of actinide elements in plutonium

January 27, 2022
Author(s)
Richard Essex, Lav Tandon, Amy Gaffney, Cole Hexel, Debbie Bostick, Lisa Colletti, Diana Decker, Casey Finstad, Joseph Giaquinito, Elmer Lujan, John Partridge, Benjamin Roach, John Rolinson, Kyle Samperton, Alice Slemmons, Khalil Spencer, Floyd Stanley, Lisa Townsend, Kerri Treinen, Ross Williams, Christopher Worley
Two plutonium oxides were prepared as unique reference materials for actinides occurring as trace constituents in Pu. Each reference material unit is approximately 200 mg of PuO2 in a quartz glass vial. Characterized attributes of the oxides included mass

Certification of Standard Reference Material(R) 2386 Avocado Powder

October 20, 2021
Author(s)
Melissa M. Phillips, Laura J. Wood, George Caceres, Grace Hahm, Karen E. Murphy, Rick L. Paul, Benjamin Place, James H. Yen
The National Institute of Standards and Technology (NIST) recently released SRM 2386 Avocado Powder which has value assignment for over 70 analytes. This material has certified values assigned for 22 analytes and non-certified values assigned for 53

Improving end-user trust in the quality of commercial probiotic products

September 24, 2021
Author(s)
Scott Jackson, Jean Schoeni, Buffy Stahl
In a rapidly growing global probiotic market, end-users have difficulty distinguishing between high quality and poor quality products. This threatens consumer and healthcare provider trust in probiotic products. To address this problem, we recommend that

Health Assessment Measurements Quality Assurance Program: Exercise 6 Final Report

September 20, 2021
Author(s)
Chuck Barber, Carolyn Burdette, Hugh Hayes, Monique Johnson, Shaun Kotoski, Jacolin Murray, Melissa M. Phillips, Kate Rimmer, Laura J. Wood, Andrea Yarberry
The Health Assessment Measurements Quality Assurance Program (HAMQAP) was launched in collaboration with the National Institutes of Health (NIH) Office of Dietary Supplements (ODS) in 2017. HAMQAP was established to enable laboratories to improve the

Assessing the Microscale Heterogeneity in Candidate Standard Reference Material 4600: Surrogate Post-detonation Urban Debris

August 18, 2021
Author(s)
Jacqueline L. Mann, John L. Molloy, Mark Tyra, Kevin Pfeuffer, Barbara Fallon, JoAnn Buscaglia
Nondestructive microbeam X-ray fluorescence (µXRF) spectrometry has been used to investigate the elemental microheterogeneity in a candidate nuclear forensics reference material (RM), NIST SRM 4600: Surrogate Post-detonation Urban Debris. Using a principal

Challenges of Accuracy in Germline Clinical Sequencing Data

July 20, 2021
Author(s)
Justin Zook, Ryan Poplin, Mark DePristo
Physicians are increasingly using clinical sequencing tests to establish diagnoses of patients who might have genetic disorders, which means that accuracy of sequencing and interpretation are important elements in ensuring the benefits of genetic testing

Reference standards for accurate validation and optimization of assays that determine integrated Lentiviral vector copy number in transduced cells

July 11, 2021
Author(s)
Barbara S. Paugh, Lajos Baranyi, Andre Roy, Hua-Jun He, Lindsay Harris, Kenneth Cole, Caroline Raimund, Patricia S. Langan, Moria Artlip, Srikanta Jana, Rimas J. Orentas, Sheng Lin-Gibson, Winfried Krueger, Boro Dropuli?
Lentiviral vectors (LV) have emerged as a robust technology for therapeutic gene delivery into human cells as advanced medicinal products. As these products are increasingly commercialized, there are concomitant demands for their characterization to ensure
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