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Bioscience

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Displaying 76 - 100 of 1242

Towards Ontologizing a Digital Twin Framework for Manufacturing

September 21, 2023
Author(s)
Milos Drobnjakovic, Guodong Shao, Ana Nikolov, Boonserm Kulvatunyou, Simon P. Frechette, Vijay Srinivasan
This paper presents a timely opportunity to ontologize a digital twin frame-work for manufacturing using recently published international standards and proposes a collaborative research effort to accomplish this task. The ISO/IEC 21838 series of standards

Multi'omic Characterization of Human Whole Stool RGTMs

September 8, 2023
Author(s)
Amanda Bayless, Sandra Da Silva, William Davis, Abraham Kuri Cruz, Tracey Johnston, Stephanie Servetas, Paulina Piotrowski
The gut microbiome plays a critical role in a vast and disparate set of health and disease states, including cancer and obesity. Human fecal is a complex mixture including microbes, proteins, undigested plant matter and fat content according to the diet

Quantitation and integrity evaluation of RNA genome in lentiviral vectors by direct reverse transcription-droplet digital PCR (direct RT-ddPCR)

September 2, 2023
Author(s)
Zhiyong He, Edward Kwee, Megan Cleveland, Kenneth Cole, Sheng Lin-Gibson, Hua-Jun He
Abstract Lentiviral vectors (LV) have proven to be powerful tools for stable gene delivery in both dividing and non-dividing cells. Approval of these LVs for use in clinical applications has been achieved by improvements in LV design. Critically important

A multipathway phosphopeptide standard for rapid phosphoproteomics assay development

August 30, 2023
Author(s)
Brian Searle, Allis Chien, Antonius Koller, David Hawke, Anthony Herren, Jenny Kim, Kimberly Lee, Ryan Leib, Alissa Nelson, Jian Min Ren, Paul Stemmer, Yiying Zhu, Ben Neely, Bhavin Patel
Recent advances in methodology have made phosphopeptide analysis a tractable problem for many proteomics researchers. There are now a wide variety of robust and accessible enrichment strategies to generate phosphoproteomes while free or inexpensive

National Institute of Standards and Technology Environmental Scan 2023: Societal and Technology Landscape to Inform Science and Technology Research

August 23, 2023
Author(s)
Ashley Russell, Kerrianne Buchanan, David Griffith, Heather Evans, Dimitrios Meritis, Lisa Ng, Anna Sberegaeva, Michelle Stephens
The 2023 National Institute of Standards and Technology Environmental Scan provides an analysis of key external factors that could impact NIST and the fulfillment of its mission in coming years. The analyses were conducted through three separate lenses

The complete sequence of a human Y chromosome

August 23, 2023
Author(s)
Arang Rhie, Sergey Nurk, Monika Cechova, Savannah Hoyt, Dylan Taylor, Nathanael David Olson, Justin Zook, Adam Phillippy
The human Y chromosome has been notoriously difficult to sequence and assemble because of its complex repeat structure that includes long palindromes, tandem repeats and segmental duplications1,2,3. As a result, more than half of the Y chromosome is

A Draft Human Pangenome Reference

May 10, 2023
Author(s)
Wen-Wei Liao, Mobin Asri, Jana Ebler, Jennifer McDaniel, Nathanael David Olson, Justin Wagner, Justin Zook, Erik Garrison, Tobias Marschall, Ira Hall, Heng Li, Benedict Paten
Here the Human Pangenome Reference Consortium presents a first draft of the human pangenome reference. The pangenome contains 47 phased, diploid assemblies from a cohort of genetically diverse individuals1. These assemblies cover more than 99% of the

Mechanical Regulation of Oral Epithelial Barrier Function

April 25, 2023
Author(s)
Eun Jin Lee, Paul Salipante, Anthony Kotula, Dana Graves, Stella Alimperti, Yoontae Kim
Epithelial cell function is modulated by mechanical forces imparted by the extracellular environment. The transmission of forces onto the cytoskeleton by modalities such as mechanical stress and matrix stiffness is necessary to address by the development

Variant calling and benchmarking in an era of complete human genome sequences

April 14, 2023
Author(s)
Nathanael David Olson, Justin Wagner, Nathan Dwarshuis, Karen Miga, Marc L. Salit, Justin Zook
Genetic variant calling from DNA sequencing has enabled understanding of germline variation in hundreds of thousands of humans. Sequencing technologies and variant-calling methods have advanced rapidly, routinely providing reliable variant calls in most of

The emerging landscape of single-molecule protein sequencing technologies

April 11, 2023
Author(s)
Zvi Kelman, John Marino
Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe

Three-dimensional, label-free cell viability measurements in tissue engineering scaffolds using optical coherence tomography

March 14, 2023
Author(s)
Greta Babakhanova, Anant Agrawal, Deepika Arora, Allison Horenberg, Jagat Budhathoki, Joy Dunkers, Joe Chalfoun, Peter Bajcsy, Carl Simon Jr.
In the field of tissue engineering, 3D scaffolds and cells are often combined to yield constructs that are used as therapeutics to repair or restore tissue function in patients. Viable cells are required to achieve the intended mechanism of action for the

Variability in genome-engineering source materials: Consider your starting point

March 10, 2023
Author(s)
Simona Patange, Sierra Miller, Samantha Maragh
The presence and impact of variability in cells as the source material for genome engineering are important to consider for the design, execution and interpretation of outcomes of a genome-engineering process. Variability may be present at the genotype and

Micelle formation and phase separation of poloxamer 188 and preservative molecules in aqueous solutions studied by small angle X-ray scattering

March 1, 2023
Author(s)
Rachel Ford, Peter H. Gilbert, Richard Gillilan, Qingqiu Huang, Roisin Donnelly, Ken Qian, David Allen, Norman Wagner, Yun Liu
Multi-injection pharmaceutical products such as insulin must be formulated to prevent aggregation and microbial contamination. Small-molecule preservatives and nonionic surfactants such as poloxamer 188 (P188) are thus often employed in protein drug
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