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The emerging landscape of single-molecule protein sequencing technologies



Zvi Kelman, John Marino


Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.
Nature Methods


Protein sequencing, proteom


Kelman, Z. and Marino, J. (2023), The emerging landscape of single-molecule protein sequencing technologies, Nature Methods (Accessed February 26, 2024)
Created April 11, 2023