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Bioscience

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Displaying 626 - 650 of 1242

Active Transcriptomic and Proteomic Reprogramming in the C. elegans Nucleotide Excision Repair Mutant xpa-1

April 9, 2013
Author(s)
Katarzyna Arczewska, Gisele Tomazella, Jessica Lindvall, Henok Kassahun, Silvia Maglioni, Alessandro Torgovnick, Johan Henriksson, Olli Matilainen, Bryce J. Marquis, Bryant Nelson, Eshrat Babaie, Carina Holmberg, Thomas Burglin, Natascia Ventura, Bernd Thiede, Hilde Nilsen
Transcription-blocking oxidative DNA damage is believed to contribute to aging and to underlie activation of oxidative stress-responses and down-regulation of insulin-like signaling (ILS) in Nucleotide Excision Repair (NER) deficient mice. Here, we present

Guidelines for Minimum Information for Publication of Quantitative Digital PCR Experiments

April 9, 2013
Author(s)
Ross J. Haynes, Jim Huggett, Carole Foy, Vladimir Benes, Kerry Emslie, Jeremy Garson, Jan Hellemans, Mikael Kubista, Tania Nolan, Michael Pfaffl, Gregory Shipley, Jo Vandesompele, Carl Wittwer, Stephen Bustin
There is growing interest in the digital polymerase chain reaction (dPCR) as technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acid, facilitating the measurement of small

Salivary Antigen-5/CAP Family Members Are Cu 2+ -dependent Antioxidant Enzymes That Scavenge O 2 -. and Inhibit Collagen-induced Platelet Aggregation and Neutrophil Oxidative Burst

April 4, 2013
Author(s)
Teresa Assumpcao, Dongying Ma, Alexandra Schwarz, Karine Reiter, Jaime Santana, John Andersen, Jose Ribeiro, Glen Nardone, Lee L. Yu, Ivo Francischetti
The function of the antigen-5/CAP family of proteins found in the salivary gland of bloodsucking animals has remained elusive for decades. Antigen-5 members from the hematophagous insects Dipetalogaster maxima (DMAV) and Triatoma infestans (TIAV) were

Spectral Light source Distribution variations to enhance discrimination of the common bile duct from surroundings in reflectance hyperspectral imaging

March 8, 2013
Author(s)
Maritoni A. Litorja, Mira Fein, Eleanor Wehner, Karel Zuzak, Edward Livingston
The classification of anatomical features using hyperspectral imaging has been a common goal in biomedical hyperspectral imaging. Identification and location of the common bile duct is critical in cholecystectomies, one of the most common surgical

Gradient Nanofiber Scaffold Libraries for Screening Cell Response to Poly(e-caprolactone)-Calcium Phosphate Composites

March 1, 2013
Author(s)
Carl G. Simon Jr., Limin Sun, Laurence C. Chow, William Miles, Christopher K. Tison, Kaushik Chatterjee, Marian F. Young, Vinoy Thomas, Murugan Ramalingam
A 2-spinnerette approach has been developed for fabricating nanofiber scaffold gradients for use as 1) scaffold libraries for screening the effect of nanofiber properties on cell response and 2) templates for generating graded tissue interfaces. Although

Standard Reference Material 2366 for Measurement of Human Cytomegalovirus DNA

March 1, 2013
Author(s)
Ross J. Haynes, Margaret C. Kline, Blaza Toman, Calum Scott, Paul Wallace, John M. Butler, Marcia J. Holden
Cytomegalovirus (CMV) is one of a number of human viruses that are ubiquitous in human populations, generally cause little illness upon infecting healthy individuals, but can cause life threatening disease in immune-compromised individuals. An important

Modification of tethered bilayers by phospholipid exchange with vesicles

February 27, 2013
Author(s)
David J. Vanderah, Rima Budvytyte, Mindaugas Mickevicius, Gintaras Valincius, Frank Heinrich
Phosphatidylcholine and cholesterol exchange between vesicles and planar tethered bilayer lipid membranes (tBLMs) was demonstrated from electrochemical impedance spectroscopy (EIS), fluorescence microscopy (FM), and neutron reflectometry (NR) data

Mouse Cell Line Authentication

February 13, 2013
Author(s)
Jamie L. Almeida, Carolyn R. Steffen, Kenneth D. Cole
The scientific community has responded to the misidentification of human cell lines with validated methods to authenticate these cells; however, there are few assays available for nonhuman cell line identification. We have developed a multiplex polymerase

Thermococcus kodakarensis DNA replication

February 2, 2013
Author(s)
Zvi Kelman, Lori Kelman, Zhuo Li
DNA replication plays an essential role in all life forms. Research on archaeal DNA replication began about twenty years ago. Progress was hindered, however, by the lack of genetic tools to supplement the biochemical and structural studies. This has

Further Studies with Isolated Absolute Infrared Spectra of Bacteriorhodopsin Photocycle Intermediates: Conformational Changes and Possible Role of a New Proton-Binding Center

January 1, 2013
Author(s)
Richard W. Hendler, Curtis W. Meuse, Paul Smith, John W. Kakareka
We recently published procedures describing the isolation of absolute infrared spectra for the intermediates of the bacteriorhodopsin (BR) photocycle and from these, obtaining transitional difference spectra between consecutive intermediates. In that work

Revision of the SNPforID 34-plex forensic ancestry test: Assay enhancements, standard reference sample genotypes and extended population studies

January 1, 2013
Author(s)
John Butler, Manuel Fondevila, Carla Santos, Ana Freire, Christopher Phillips, M Lareu, Peter Vallone, A. Carracedo
A revision of an established 34 SNP forensic ancestry test has been made by swapping the under- performing rs727811 component SNP with the highly informative rs3827760 that shows a near-fixed East Asian specific allele. We collated SNP variability data for

Identification and Quantification of Human DNA Repair Protein NEIL1 by Liquid Chromatography/Isotope-Dilution Tandem Mass Spectrometry

December 26, 2012
Author(s)
M Miral Dizdar, Pawel Jaruga, Guldal Kirkali, Bryant C. Nelson, Gamze Tuna, Prasad T. Reddy
Accumulated evidence points to DNA repair capacity as an important factor in cancer and other diseases. DNA repair proteins are promising drug targets and are emerging as prognostic and therapeutic biomarkers. Thus, the knowledge of the overexpression or

Structure and Activity of PA5508, a Novel Hexameric Glutamine Synthase Homolog

December 12, 2012
Author(s)
Jane E. Ladner, James F. Parsons
The structure of PA5508 from Pseudomonas aeruginosa, a glutamine synthase (GS) homolog, has been solved at 2.5 Å. Surprisingly, PA5508 forms single hexameric rings rather than the stacked double rings that are characteristic of GS. The C-terminal helical

The Effects of Diffusion on an Exonuclease/Nanopore-Based DNA Sequencing Engine

December 7, 2012
Author(s)
Joseph E. Reiner, Joseph W. Robertson, Arvind Balijepalli, Daniel L. Burden, Bryon S. Drown, John J. Kasianowicz
The ability to electronically detect and characterize individual polynucleotides as they are driven through a single protein ion channel may eventually prove useful for rapidly sequencing DNA (base-by-base) in a ticker tape-like fashion. More recently, a

Relative Rigidity of Cell-Substrate Affects Hepatic and Hepatocellular Carcinoma Cell Migration

December 3, 2012
Author(s)
Martin Y. Chiang, Hongbing Wang, Li Zhong, Qiaoyan Tan, Gurinder K. Singh, Song Li, Li Yang, Yanzi Yangben
Polyacrylamide gels with different stiffness and glass were employed as substrates to investigate how substrate stiffness affects the cellular stiffness of adherent hepatocellular carcinoma (HCCLM3) and hepatic (L02) cells. The interaction of how cell

Immobilization of Proteins on Carboxylic Acid-Functionalized Nanopatterns

November 28, 2012
Author(s)
John Peter Ngunjiri, Daniel J. Stark, Tian Tian, Kimberly Briggman, Jayne C. Garno
The immobilization of proteins on nanopatterned surfaces was investigated using in situ atomic force microscopy (AFM) and ex situ infrared reflectance-absorption spectroscopy (IRAS). The AFM-based lithography technique of nanografting provided control of
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