Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES

Published

Author(s)

Conni Hanke, Petra S. Dittrich, Darwin Reyes-Hernandez

Abstract

A cell co-culture was generated on opposite sides of a permeable polyester membrane (PET) membrane by combining dielectrophoretic (DEP) and electrostatic forces to hold cells against gravity in a multilayer microfluidic device (Figure 1). HepG2 cells were trapped underneath the PET membrane (bottom channel) when DEP attractive forces were applied. Cells immediately adhered on the PET membrane by the electrostatic forces from a hybrid cell adhesive material (hCAM) deposited on the surface of the electrodes. NIH-3T3 cells adhered on the top channel via the hCAM deposited on that side of the membrane. Cells on both sides of the membrane remained attached and viable at least 24 h after the co-culture was generated.
Proceedings Title
Proceedings of Micro Total Analysis Systems 2012
Conference Dates
October 28-November 1, 2012
Conference Location
Okinawa, JP
Conference Title
The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Keywords

Dielectrophoresis, cell co-cultures, polyester (PET) membrane, fibronectin, polyelectrolyte multilayers

Citation

Hanke, C. , Dittrich, P. and Reyes-Hernandez, D. (2012), GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES, Proceedings of Micro Total Analysis Systems 2012, Okinawa, JP (Accessed February 21, 2024)
Created October 27, 2012, Updated October 12, 2021