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The Cell-Free Workshop sought to identify and prioritize actionable steps towards more reproducible and comparable cell-free systems for practical applications in bioengineering and biomanufacturing.


Stakeholders of the National Institute of Standards and Technology (NIST) in industry, government, and academia have requested measurement tools and methods to improve reproducibility, primarily for protein expression using cell-free systems, to support intra- and interlaboratory collaboration, assist nascent industry, and improve fundamental understanding of biology. As part of a broader portfolio of investments in synthetic biology and engineering biology, NIST is building technical competence and measurement capabilities in cell-free systems.

Towards reproducible cell-free systems
Credit: Elizabeth Strychalski

Major Accomplishments

Created June 19, 2019, Updated February 26, 2020