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Eugenia (Jane) Romantseva (Fed)
Engineer
Eugenia (Jane) Romantseva is a staff engineer supporting the Cellular Engineering Group. Her current work focuses on developing measurement assurance for cell-free expression systems to promote reproducibility, best practices, and standards. Jane is also part of the collaborative effort to leverage automation, specifically the NIST Living Measurement Systems Foundry, for (1) high throughput measurements of genotype-phenotype landscapes for genetic sensor engineering, (2) rapid prototyping of engineering biological parts to speed innovation in synthetic biology workflows, (3) developing measurement assurance for predictable engineering with living systems on a commercial scale.
HIGHLIGHTED Publications
Romantseva, E. , Piorino Macruz de Oliveira, F. , Sundberg, C. , Sittampalam, G. and Strychalski, E. (2024), Advancing Cell-Free Manufacturing: Challenges in Scale-up and Automation Workshop Report, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, https://doi.org/10.6028/NIST.SP.1500-26
Engineering Biology Research Consortium (2024). Engineering Biology for Space Health: An Innovative Research Roadmap. Retrieved from http://roadmap.ebrc.org. https://doi.org/10.25498/E4D59R
Babakhanova G, Simon, Jr. CG, Romantseva E (2024) Measurement Needs for Biofabrication of Tissue Engineered Medical Products Workshop Report. (National Institute of Standards and Technology, Gaithersburg, MD), NIST Special Publication 1500 (SP) NIST SP 1500-23. https://doi.org/10.6028/NIST.SP.1500-23
Freemont, P.S., Ni, C., Aurand, E., Chang, M.W., Hook-Barnard, I., Malley, J., Romantseva, E., Strychalski, E., Vavitsas, K. 2024. Engineering Biology Metrics and Technical Standards for the Global Bioeconomy. London, UK https://doi.org/10.25561/110822.
Eugenia Romantseva, Nina Alperovich, David Ross, Steven P Lund, Elizabeth A Strychalski, Effects of DNA template preparation on variability in cell-free protein production, Synthetic Biology, Volume 7, Issue 1, 2022, ysac015, https://doi.org/10.1093/synbio/ysac015
Garenne, D., Haines, M.C., Romantseva, E.F., Freemont P., Strychalski E.A., Noireaux V. Cell-free gene expression. Nat Rev Methods Primers 1, 49 (2021). https://doi.org/10.1038/s43586-021-00046-x
Romantseva J, Ross D, Alperovich A, Strychalski E, Automated DNA template preparation and quantitation methods, https://www.protocols.io, March 23, 2022, https://dx.doi.org/10.17504/protocols.io.j8nlkk8rdl5r/v1
Romantseva E.F., Tack D.S., Alperovich N., Ross D., Strychalski E.A. (2022) Best Practices for DNA Template Preparation Toward Improved Reproducibility in Cell-Free Protein Production. In: Karim A.S., Jewett M.C. (eds) Cell-Free Gene Expression. Methods in Molecular Biology, vol 2433. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1998-8_1
“The genotype-phenotype landscape of an allosteric protein” D. S. Tack, P. D. Tonner, A. Pressman, N. D. Olson, S. F. Levy, E. F. Romantseva, N. Alperovich, O. Vasilyeva, D. Ross, Molecular Systems Biology, Vol. 17, e10179, 2021. https://doi.org/10.15252/msb.202010179
“Measurements drive progress in directed evolution for precise engineering of biological systems” D.S. Tack, E. F. Romantseva, P. D. Tonner, A. Pressman, J. Rammohan, E. A. Strychalski, Current Opinion in Systems Biology, Vol. 23, pp. 32-37, 2020. https://doi.org/10.1016/j.coisb.2020.09.004
“CELL-FREE (Comparable Living Lysates For Research, Education, and Entrepreneurship) workshop Report” E. Romantseva, E. A. Strychalski, https://nvlpubs.nist.gov/nistpubs/SpecialPublications/NIST.SP.1500-13.pdf
Cybersecurity for biopharmaceutical products” J. L. Mantle, J. Rammohan, E. F. Romantseva, J. T. Welch, L. R. Kauffman, J. McCarthy, J. Schiel, J. C. Baker, E. A. Strychalski, K. C. Rogers, K. H. Lee, Frontiers in Bioengineering and Biotechnology, Vol. 7, pp. 116-122, 2019. https://doi.org/10.3389/fbioe.2019.00116
“Proteomic analysis of nuclei dissected from fixed rat brain tissue using expression microdissection” A. R. Blackler, N. Y. Morgan, B. Gao, L. R. Olano, M. D. Armani, E. Romantseva, J. W. Kakareka, R. F. Bonner, S. Mukherjee, B. Xiao, K. Tran, T. J. Pohida, M. R. Emmert-Buck, M. A. Tangrea, S. P. Markey, Analytical Chemistry, Vol. 85, No. 15, pp. 7139-7145, 2013
“Gradient elution moving boundary electrophoresis with channel current detection” D. Ross, E. F. Romantseva, Analytical Chemistry, Vol. 81, No. 17, pp. 7326-7335, 2009. https://doi.org/10.1021/ac901189y