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Eugenia (Jane) Romantseva (Fed)


Eugenia (Jane) Romantseva is a staff engineer supporting the Cellular Engineering Group. Her current work focuses on developing measurement assurance for cell-free expression systems to promote reproducibility, best practices, and standards. Jane is also part of the collaborative effort to leverage automation, specifically the NIST Living Measurement Systems Foundry, for (1) high throughput measurements of genotype-phenotype landscapes for genetic sensor engineering, (2) rapid prototyping of engineering biological parts to speed innovation in synthetic biology workflows, (3) developing measurement assurance for predictable engineering with living systems on a commercial scale.

HIGHLIGHTED Publications 

Eugenia Romantseva, Nina Alperovich, David Ross, Steven P Lund, Elizabeth A Strychalski, Effects of DNA template preparation on variability in cell-free protein production, Synthetic Biology, Volume 7, Issue 1, 2022, ysac015,

Garenne, D., Haines, M.C., Romantseva, E.F., Freemont P., Strychalski E.A., Noireaux V.  Cell-free gene expression. Nat Rev Methods Primers 1, 49 (2021).

Romantseva J, Ross D, Alperovich A, Strychalski E, Automated DNA template preparation and quantitation methods,, March 23, 2022,

Romantseva E.F., Tack D.S., Alperovich N., Ross D., Strychalski E.A. (2022) Best Practices for DNA Template Preparation Toward Improved Reproducibility in Cell-Free Protein Production. In: Karim A.S., Jewett M.C. (eds) Cell-Free Gene Expression. Methods in Molecular Biology, vol 2433. Humana, New York, NY.

“The genotype-phenotype landscape of an allosteric protein” D. S. Tack, P. D. Tonner, A. Pressman, N. D. Olson,  S. F. Levy, E. F. Romantseva, N. Alperovich, O. Vasilyeva, D. Ross, Molecular Systems Biology, Vol. 17, e10179, 2021

“Measurements drive progress in directed evolution for precise engineering of biological systems” D.S. Tack, E. F. Romantseva, P. D. Tonner, A. Pressman, J. Rammohan, E. A. Strychalski, Current Opinion in Systems Biology, Vol. 23, pp. 32-37, 2020

“CELL-FREE (Comparable Living Lysates For Research, Education, and Entrepreneurship) workshop Report” E. Romantseva, E. A. Strychalski,

Cybersecurity for biopharmaceutical products” J. L. Mantle, J. Rammohan, E. F. Romantseva, J. T. Welch, L. R. Kauffman, J. McCarthy, J. Schiel, J. C. Baker, E. A. Strychalski, K. C. Rogers, K. H. Lee, Frontiers in Bioengineering and Biotechnology, Vol. 7, pp. 116-122, 2019

“Proteomic analysis of nuclei dissected from fixed rat brain tissue using expression microdissection” A. R. Blackler, N. Y. Morgan, B. Gao, L. R. Olano, M. D. Armani, E. Romantseva, J. W. Kakareka, R. F. Bonner, S. Mukherjee, B. Xiao, K. Tran, T. J. Pohida, M. R. Emmert-Buck, M. A. Tangrea, S. P. Markey, Analytical Chemistry, Vol. 85, No. 15, pp. 7139-7145, 2013

“Gradient elution moving boundary electrophoresis with channel current detection” D. Ross, E. F. Romantseva, Analytical Chemistry, Vol. 81, No. 17, pp. 7326-7335, 2009


The allosteric landscape of the lac repressor

Drew S. Tack, Peter Tonner, Abe Pressman, Nathanael David Olson, Eugenia Romantseva, Nina Alperovich, Olga Vasilyeva, David J. Ross, Sasha F. Levy
Allostery is a fundamental biophysical mechanism where the activity of a biomolecule is regulated by the binding of a ligand. Despite playing a central role in

Cyberbiosecurity for biopharmaceutical products

Elizabeth Strychalski, Jennifer Mantla, Jayan Rammohan, Eugenia Romantseva, Reid D'Amico, Joel Welch, Leah R. Kauffman, James McCarthy, John E. Schiel, Jeffrey Baker, Kelley Rogers, Kelvin Lee
Created October 2, 2019, Updated February 6, 2023