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Joy Dunkers (Fed)

Physical Scientist

 Advanced Microscopy of Complex Microbial Communities

This project supports the Microbial Metrology program to advance microbiome community measurements and pathogen detection and identification. It uses advanced optical imaging methods and associated data analysis tools to address shortcomings in the quality, quantity or specificity of information acquired from conventional microbial methods to help answer the following critical questions: Who are they? Where are they? How many are here? What are they doing?

We are developing novel microbial fluorescence in situ hybridization (FISH) techniques, novel cell shape metric and viability measurements to advance the following projects:

  • 3D Structure-Function Relationships of Complex Microbial Communities using Multiplexed FISH
  • Whole Cell Genome Copy Number Validation
  • Absolute Measurements of Microbial Viability

 

Research Opportunities

NIST-NRC Postdoctoral Research Associateship Program: 2-year fellowship at NIST, US citizens only, $74,750 salary plus benefits, travel stipend and relocation expenses. Requires 10 page research proposal plus additional materials. Contact me if interested in writing a microbial research proposal. Application deadlines are Feb. 1 and Aug. 1

Current opportunities available:

 

Mentoring

Primary or co-advisor for 5 NRC postdocs

Advisor to 2 Montgomery College undergraduate students

 

Awards and Funding

Material Measurement Laboratory Collaboration and Teamwork Accolade for assisting the Office of Advanced Manufacturing as subject matter experts advising NIIMBL project teams funded through the CARES Act to respond to the pandemic national emergency, 2021

Co-champion of Innovations in Measurement Science Award for Shape Metrology Proposal, 2011-2013

Department of Commerce Gold Medal for Distinguished Achievement in Federal Service for Creating and Implementing Rigorous Testing Protocols and Benchmarks Needed to Ensure the Security and Integrity of the New US ePassport, 2007

Secured Phase I ($75k) and Phase II ($270k) of a Small Business Innovative Research award to develop a high speed dual channel imaging microscope for deep imaging of cells, tissue and tissue engineered constructs using optical coherence microscopy and two photon fluorescence, 2006-2008

 

Professional Organizations

American Society for Microbiology (National and DC Chapters)
 

Publications

Towards Absolute Viability Measurements for Bacteria

Author(s)
Joy Dunkers, Hariharan K. Iyer, Brynna H. Jones, Charles Camp, Stephan J. Stranick, Nancy Lin
Quantifying viable, vegetative bacteria is a critical measurand in healthcare diagnostics, food safety, and antimicrobial development. Viability determination

Key Considerations for Microbial Viability Measurements

Author(s)
Joy Dunkers, Sandra M. Da Silva, Stephanie Servetas, James J. Filliben, Guilherme L. Pinheiro, Nancy Lin
Making reliable measurements of antimicrobial killing efficacy requires careful consideration of the sources of biological variability, measurement bias and
Created July 30, 2019, Updated July 11, 2022