David P. Hoogerheide

Instrument scientist for the CANDoR white beam reflectometer and a lead scientist on the ROADMAP project

Interests:

Applications of neutron scattering to biological materials
Novel platforms for neutron reflectivity measurements of membrane proteins
Structure-function relationships of membrane active peptides (ROADMAP)
Single-molecule methods for probing membrane proteins
Energy landscape modeling for nanopore capture of biopolymers

Selected Publications

Neutron Scattering for Structural Biology

June 1, 2020

Author(s)

David P. Hoogerheide, V. Trevor Forsyth, Katherine A. Brown

Structural Investigations of Membrane-Associated Proteins by Neutron Reflectometry

July 1, 2019

Author(s)

Rebecca Eells, David Hoogerheide, Paul A. Kienzle, Mathias Loesche, Charles Majkrzak, Frank Heinrich

Neutron reflectometry is a powerful technique for probing the structure of lipid bilayer membranes and membrane-associated proteins. Measurements of the

Effect of a Post-Translational Modification Mimic on Protein Translocation through a Nanopore

April 27, 2020

Author(s)

David P. Hoogerheide, Philip A. Gurnev, Tatiana K. Rostovtseva, Sergey M. Bezrukov

Post-translational modifications (PTMs) of proteins are recognized as crucial components of cell signaling pathways through modulating folding, altering

Structural Features and Lipid Binding Domain of Tubulin on Biomimetic Mitochondrial Membranes

April 18, 2017

Author(s)

David Paul Hoogerheide, Sergei Y. Noskov, Daniel Jacobs, Lucie Bergdoll, Vitalii Ivanovich Silin, David L. Worcester, Jeff Abramson, Hirsh Nanda, Tatiana K. Rostovtseva, Sergey M. Bezrukov

Amphitropic proteins, the subfamily of peripheral membrane proteins that interact directly with lipid bilayers, comprises members whose function depends on

Publications

Restricting α-Synuclein Transport into Mitochondria by Inhibition of α-Synuclein-VDAC Complexation as a Potential Therapeutic Target for Parkinson's Disease Treatment

October 1, 2022

Author(s)

Megha Rajendran, Maria Queralt-Martin, Philip A. Gurnev, William M. Rosencrans, Amandine Rovini, Daniel Jacobs, Kaitlin Abrantes, David Hoogerheide, Sergey M. Bezrukov, Tatiana Rostovtseva

Involvement of alpha-synuclein (αSyn) in Parkinson's disease (PD) is complicated and difficult to trace on cellular and molecular levels. Recently we

The Single Residue K12 Governs the Exceptional Voltage Sensitivity of Mitochondrial Voltage-Dependent Anion Channel Gating

August 17, 2022

Author(s)

Van Ngo, Maria Queralt-Martin, Farha Khan, Lucie Bergdoll, Jeff Abramson, Sergey M. Bezrukov, Tatiana Rostovtseva, David Hoogerheide, Sergei Noskov

The voltage-dependent anion channel (VDAC) is a β-barrel channel of the mitochondrial outer membrane (MOM) that passively transports ions, metabolites

Voltage-Activated Complexation of α-Synuclein with Three Diverse β-Barrel Channels: VDAC, MspA, and α-Hemolysin

March 1, 2022

Author(s)

David Hoogerheide, Philip A. Gurnev, Tatiana Rostovtseva, Sergey M. Bezrukov

Voltage-activated complexation is the process by which a transmembrane potential drives complex formation between a membrane-embedded channel and a soluble or

Low-Background Neutron Reflectometry from Solid/Liquid Interfaces

February 1, 2022

Author(s)

David Hoogerheide, Joseph Dura, Brian B. Maranville, Charles F. Majkrzak

Liquid cells are an increasingly common sample environment for neutron reflectometry experiments and are critical for measuring the properties of materials at

Exploring Lipid-Dependent Conformations of Membrane-Bound α-Synuclein with the VDAC Nanopore

September 1, 2021

Author(s)

David Hoogerheide, Tatiana Rostovtseva, Sergey M. Bezrukov

Regulation of VDAC by α-Synuclein (αSyn) is a rich and instructive example of protein-protein interactions catalyzed by a lipid membrane surface. αSyn, a

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2010-2013	Harvard University Postdoctoral Fellow (Jene Golovchenko)
2013-2015	NIH/NIST Joint Research Associateship (National Research Council)
2015-present	NIST Center for Neutron Research

2004	B. S., Physics and Chemistry	Western Michigan University
2006	A. M., Physics	Harvard University (advisor: Jene Golovchenko)
2010	Ph. D., Physics	Harvard University (advisor: Jene Golovchenko)

2010-2013	Harvard University Postdoctoral Fellow (Jene Golovchenko)
2013-2015	NIH/NIST Joint Research Associateship (National Research Council)
2015-present	NIST Center for Neutron Research

2004	B. S., Physics and Chemistry	Western Michigan University
2006	A. M., Physics	Harvard University (advisor: Jene Golovchenko)
2010	Ph. D., Physics	Harvard University (advisor: Jene Golovchenko)

David P. Hoogerheide (Fed)

Physicist

Selected Publications

Neutron Scattering for Structural Biology

Structural Investigations of Membrane-Associated Proteins by Neutron Reflectometry

Effect of a Post-Translational Modification Mimic on Protein Translocation through a Nanopore

Structural Features and Lipid Binding Domain of Tubulin on Biomimetic Mitochondrial Membranes

Publications

Restricting α-Synuclein Transport into Mitochondria by Inhibition of α-Synuclein-VDAC Complexation as a Potential Therapeutic Target for Parkinson's Disease Treatment

The Single Residue K12 Governs the Exceptional Voltage Sensitivity of Mitochondrial Voltage-Dependent Anion Channel Gating

Voltage-Activated Complexation of α-Synuclein with Three Diverse β-Barrel Channels: VDAC, MspA, and α-Hemolysin

Low-Background Neutron Reflectometry from Solid/Liquid Interfaces

Exploring Lipid-Dependent Conformations of Membrane-Bound α-Synuclein with the VDAC Nanopore