Projects/Programs

Displaying 1 - 14 of 14

Biofabrication of Tissue Engineered Constructs

Ongoing

OCT imaging was assessed using a model scaffold-cell system consisting of a polysaccharide-based hydrogel seeded with human Jurkat cells. Four test systems were used: hydrogel seeded with live cells, hydrogel seeded with heat-shocked or fixed dead cells and hydrogel without any cells. Time series

Cell Viability for Advanced Therapies

Ongoing

In the Biosystems and Biomaterials Division at NIST, we have established several technical programs to support the development and implementation of cell viability assays in advanced therapies. These programs address key needs in cell viability assay development including selection and development

Development of the NIST Prototype Advanced Biometrology Laboratory

Ongoing

Currently, NIST is investing in a new type of metrology laboratory that is focused on the unique reference material and high quality assay protocols that are needed in the regenerative medicine and biotechnology communities. The laboratory design is centered on automation that can set up 1000’s of

Engineering of G Protein-Coupled Receptors

Ongoing

A trait of all life is the ability to sense and respond to changing environments, and the largest family of eukaryotic proteins that sense and respond to extracellular signals are called G Protein-Coupled Receptors (GPCRs). In humans, over 800 GPCRs detect a wide range of biological and chemical

LANTERN - Interpretable modeling of genotype-phenotype landscapes with state-of-the-art predictive power

Ongoing

LANTERN: genotype-phenotype LANdscape in TERpretable Non-parametric model The bioeconomy is increasingly data-driven, relying on large-scale measurements to rapidly engineer novel functions. To facilitate rational engineering, where complex designs are constructed from well understood components

Methods to determine the evolutionary stability of engineered biological function

Ongoing

Advances in engineering biology have greatly expanded our ability to add new and useful functions to living cells. Engineering stable functions has remained a significant challenge, with many different approaches developed to increase the stability, or decrease the variability, of a function over

NIST Living Measurement Systems Foundry

Ongoing

To enable the production of high-throughput, high-quality data that meets the rapidly evolving needs of current and future stakeholders, NIST has established an automation facility for the growth, manipulation, sample preparation, and measurement of engineered microbes. The core of the facility

NISTCHO

Ongoing

NISTCHO, a new mammalian cell line of interest to the biopharmaceutical and biomanufacturing communities, is now available from the National Institute of Standards and Technology. This Chinese hamster ovary (CHO) cell line has been engineered by MilliporeSigma to express a non-originator version of

Prototype Cell Assay Measurement Platform (P-CAMP)

Ongoing

A systematic approach to the development of robust biological assay includes identifying and ranking the sources of variability in an assay protocol. With a focus on mammalian cellular assays, the Prototype Cell Assay Measurement Platform ( P-CAMP) laboratory was designed and built to take advantage

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Ongoing

The current gold standard for cell viability measurements is the trypan blue assay. Trypan blue dye enters dead cells that have a ruptured membrane. As a result, the dead cells are stained blue. Live cells with an intact membrane, on the other hand, do not absorb the dye molecules and remain

RNA Computation and Metrology for Engineering Biology

Ongoing

RNA computation RNA computation, i.e., programmed interactions between RNA molecules that process information, is an increasingly useful synthetic biology tool with applications spanning in vitro biosensing, synthetic cells, and cellular control. We have developed cotranscriptionally encoded RNA

Serology and Neutralization Assays for COVID-19

Ongoing

Multiplexed bead-based SARS-CoV-2 serological assay We have developed a validated multiplexed bead-based SARS-CoV-2 serological assay that measures different antibodies produced by a patient (IgG, IgM, IgA). In collaboration with the NIST Applied and Computational Mathematics Division, we have

Single-molecule biosensors

Ongoing

We are developing new tools to investigate the energy landscape of single-molecule sensors. Our goal is to apply a wide-ranging array of technologies to probe the critical physicochemical properties of nanopore biosensors. These properties include the free energy of confinement for polymers as they

Synthetic Biology

Ongoing

New measurement methods and tools are required for biology to emerge fully as an enabling, practical platform for engineering. The Cellular Engineering Group works to provide a foundation of measurement assurance to support the control and rational design of biological function. Through state of the

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