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Search Publications by: Zhiyong He (Fed)

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Displaying 1 - 5 of 5

Intermethod Characterization of Commercially Available Extracellular Vesicles as Reference Materials

December 30, 2025
Author(s)
Sumeet Poudel, Diane Nelson, Blaza Toman, Zhiyong He, Ashley Green, Thomas Cleveland, Sean Lehman, Wyatt Vreeland, Kurt Benkstein, Bryant Nelson, Lili Wang, Elzafir Elsheikh, Yuefan Wang, Hui Zhang
The National Institute of Standards and Technology (NIST) is developing analytical methods to characterize extracellular vesicles (EVs) to support the urgent need for standardized EV reference materials (RMs). This study used orthogonal techniques

Gene Copy Number Dictates Extracellular Vesicle Cargo

June 7, 2025
Author(s)
Sumeet Poudel, Zhiyong He, Jerilyn Izac, Lili Wang
Extracellular vesicles (EVs) are membrane-surrounded vesicles that carry heterogeneous cellular components, including proteins, nucleic acids, lipids, and metabolites. EVs' intravesicular and surface contents possess many biomarkers of physiological and

Interlaboratory Assessment of Candidate Reference Materials for Lentiviral Vector Copy Number and Integration Site Measurements

April 21, 2025
Author(s)
Hua-Jun He, Zhiyong He, Steven Lund, Barbara Paugh, Jennifer McDaniel, Justin Zook, Sierra Miller, Samantha Maragh, Simona Patange, Mahir Mohiuddin, Alessandro Tona, John Elliott, Kenneth Cole, Sheng Lin-Gibson
Reference materials are essential for accurately measuring integrated lentiviral vector (LV) copy number (VCN) and integration sites for the safety and efficacy of lentivirus-based gene therapy. We conducted an interlaboratory study on NIST candidate

Quantitation and integrity evaluation of RNA genome in lentiviral vectors by direct reverse transcription-droplet digital PCR (direct RT-ddPCR)

September 2, 2023
Author(s)
Zhiyong He, Edward Kwee, Megan Cleveland, Kenneth Cole, Sheng Lin-Gibson, Hua-Jun He
Abstract Lentiviral vectors (LV) have proven to be powerful tools for stable gene delivery in both dividing and non-dividing cells. Approval of these LVs for use in clinical applications has been achieved by improvements in LV design. Critically important
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