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Search Publications by: Zhiyong He (Fed)

Displaying 1 - 2 of 2

Immortalization of Human Primary CD8+ T cells by Inserting a Single Copy of Human Telomerase Reverse Transcriptase via CRISPR/Cas9

April 25, 2025

Author(s)

Zhiyong He, Kenneth Cole, Hua-Jun He

Background: Existing human cell immortalization methods made the cells obtain oncogenesis phenotype and/or caused the cells gain and/or lose chromosomes. Immortalized normal human T cells lines provide critical in vitro models for basic research and

Quantitation and integrity evaluation of RNA genome in lentiviral vectors by direct reverse transcription-droplet digital PCR (direct RT-ddPCR)

September 2, 2023

Author(s)

Zhiyong He, Edward Kwee, Megan Cleveland, Kenneth Cole, Sheng Lin-Gibson, Hua-Jun He

Abstract Lentiviral vectors (LV) have proven to be powerful tools for stable gene delivery in both dividing and non-dividing cells. Approval of these LVs for use in clinical applications has been achieved by improvements in LV design. Critically important

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