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Search Publications by: Linhua Tian (Fed)

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Displaying 1 - 9 of 9

Standardization of Flow Cytometric Detection of Antigen Expression

January 25, 2024
Author(s)
Linhua Tian, Aaron Nelson, Tyler Lowe, Linda Weaver, Constance Yuan, Paul DeRose, Maryalice Stetler-Stevenson, Lili Wang
Background: While response to antigen-based immunotherapy relies upon the level of antigen expression by tumor cells and as decreasing levels of antigen expression can be an early indicator of developing resistance to therapy, we have developed an antigen

Monoclonal Antibodies as SARS-CoV-2 Serology Standards: Experimental Validation and Broader Implications for Correlates of Protection

October 28, 2023
Author(s)
Lili Wang, Paul Patrone, Anthony Kearsley, Jerilyn Izac, Adolfas Gaigalas, John Prostko, Hang Xie, Linhua Tian, Elzafir Elsheikh, Edward Kwee, Troy Kemp, Simon Jochum, Natalie Thornburg, Clifford McDonald, Adi Gundlapalli, Sheng Lin-Gibson
COVID-19 has highlighted the need for more accurate and reproducible measurements of humoral immunity, including antibody levels and neutralization potential, which are critical for diagnostics, seroprevalence estimates, and development of vaccines and

Size and Number Concentration Measurements of Polystyrene Nanoparticle Suspensions using Several Different Techniques

September 8, 2022
Author(s)
Paul DeRose, Kurt D. Benkstein, Elzafir B. Elsheikh, Adolfas K. Gaigalas, Sean Lehman, Dean Ripple, Linhua Tian, Wyatt Vreeland, Adam York, Yu-Zhong Zhang, Hao-Wei Wang
The number concentrations of nominal 100 nm, 200 nm and 500 nm diameter, fluorescently-labeled polystyrene nanosphere suspensions were measured using seven different techniques. Diameter values were also measured where possible. The diameter values were

Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19

March 8, 2021
Author(s)
Linhua Tian, Elzafir B. Elsheikh, Paul Patrone, Anthony Kearsley, adolfas Gaigalas, Sarah L. Inwood, Sheng Lin-Gibson, Dominic Esposito, Lili Wang
Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow

Expanding NIST's Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles

September 15, 2020
Author(s)
Paul DeRose, Linhua Tian, Elzafir B. Elsheikh, Aaron Urbas, Yu-Zhong Zhang, Hao-Wei Wang
NIST, NIH and other industry stakeholders have been working together to enable fluorescence intensities of flow cytometer calibration beads to be assigned quantitative 'ERF' values with high accuracy and precision. The ultimate goal of this effort is to

An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes

September 2, 2020
Author(s)
Deepa Rajagopal, Linhua Tian, Shiqiu Xiong, Hao-Wei Wang, Jonathan Campbell, Luisa Saraiva, S Vessillier
Activation of T lymphocytes leads to differentiation, characterized by the ability to induce production of cytokines. Accurate determination of cellular subsets that secrete particular cytokine(s) is therefore, a significant parameter for functional
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