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Search Publications by: Nathanael David Olson (Fed)

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Displaying 26 - 36 of 36

Occurrence of Antibiotic Residues in Differentially Treated Wastewater and in Reclaimed Water Utilized in Spray Irrigation

June 21, 2017
Author(s)
Prachi Kulkarni, Nathanael David Olson, Greg Raspanti, Rachel F. Rosenberg Goldstein, Shawn G. Gibbs, Amir Sapkota, Amy Sapkota
The objective of this study was to evaluate concentrations of antibiotics in differentially treated wastewater and reclaimed water. Wastewater (from several treatment processes), final effluent and reclaimed water samples (n=72) from four U.S. wastewater

PEPR: Pipeline for Evaluating Prokaryotic References

April 1, 2016
Author(s)
Nathanael D. Olson, Justin M. Zook, Daniel V. Samarov, Scott A. Jackson, Marc L. Salit
The rapid adoption of microbial whole genome sequencing in public health, clinical testing, and forensic labo-ratories requires the use of validated and well characterized measurement processes. Reference materials thatare well characterized and

THE PROFICIENCY TEST (PILOT) REPORT OF THE GLOBAL MICROBIAL IDENTIFIER INITIATIVE YEAR 2014

March 1, 2016
Author(s)
Nathanael D. Olson, Rene S. Hendriksen, Susanne K. Pedersen, Mette V. Larsen, Jamie N. Pedersen, Oksana Lukjancenko, Rolf S. Kaas, Pimlapas Leekitcharoenphon, Lasse Bergmark, Inge M. Hansen, Vitali Sintchenko, William J. Wolfgang, Henrik T. Westh, Jacob Moran-Gilad, Isabel Cuesta, Angel Zaballos, Brian Beck, Anthony Underwood, Errol Strain, James Pettengill

Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae

January 24, 2016
Author(s)
Sandra M. Da Silva, Lindsay Harris, Nathanael David Olson, Steven Lund, Autumn S. Downey, Zvi Kelman, Marc L. Salit, Jayne D. Morrow
Aims: We describe the development and interlaboratory study of modified Saccharomyces cerevisiae as a candidate material to evaluate a full detection workflow including DNA extraction and quantitative polymerase chain reaction (qPCR). Methods and results

Best Practices for Evaluating Single Nucleotide Variant Calling Methods for Microbial Genomics

July 7, 2015
Author(s)
Nathanael D. Olson, Steven P. Lund, Rebecca Colman, Jeffery T. Foster, Jason W. Sahl, James M. Schupp, Paul Keim, Jayne B. Morrow, Marc L. Salit, Justin M. Zook
Innovations in sequencing technologies have allowed biologists to make incredible advances in understanding biological systems. As experience grows, researchers increasingly recognize that analyzing the wealth of data provided by these new sequencing

International Interlaboratory Study Comparing Single Organism 16S rRNA Sequencing Data: Going Beyond Consensus Sequence Comparisons

March 5, 2015
Author(s)
Nathanael D. Olson, Justin M. Zook, Steven P. Lund, Fabiola Rojas-Cornejo, Jim Huggett, Jayne B. Morrow
The Consultative Committee for Amount of Substance established a working group to advance the metrology of microbial identification and quantification. This study represents the initial step towards international comparability and measurement assurance for