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Search Publications by: Zvi Kelman (Fed)

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Displaying 26 - 31 of 31

Thermococcus kodakarensis DNA replication

February 2, 2013
Author(s)
Zvi Kelman, Lori Kelman, Zhuo Li
DNA replication plays an essential role in all life forms. Research on archaeal DNA replication began about twenty years ago. Progress was hindered, however, by the lack of genetic tools to supplement the biochemical and structural studies. This has

Formation of dAMP-glycerol and dAMP-Tris derivatives by the Thermococcus kodakaraensis DNA primase

May 11, 2012
Author(s)
Zvi Kelman, John P. Marino, Jerard Hurwitz, Wiebke Chemnitz Galal, Miao Pan, Gary Giulian, Wei Yuan, Shuwei Li
In the presence of dATP, glycerol and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis (Tk) catalyzed the formation of two products that were identified as dAMP-glycerol and dAMP-Tris. These products were formed by the Tk p41 catalytic

ATP hydrolysis and DNA binding confer thermostability on the MCM helicase

February 25, 2009
Author(s)
Nozomi Sakakibara, Frederick P. Schwarz, Zvi Kelman
Minichromosome maintenance (MCM) helicase is the replicative helicase in archaea. The enzyme utilizes the energy derived from ATP hydrolysis to translocate along one strand of the DNA and unwind the complementary strand. Here, the effect of ATP and DNA on
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