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Search Publications by: David J. Ross (Fed)

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Displaying 1 - 25 of 52

The allosteric landscape of the lac repressor

April 13, 2022
Drew S. Tack, Peter Tonner, Abe Pressman, Nathanael David Olson, Eugenia Romantseva, Nina Alperovich, Olga Vasilyeva, David J. Ross, Sasha F. Levy
Allostery is a fundamental biophysical mechanism where the activity of a biomolecule is regulated by the binding of a ligand. Despite playing a central role in many biological processes, a quantitative understanding of allostery is lacking. To

A Roadmap for LIMS at NIST Material Measurement Laboratory

April 11, 2022
Gretchen Greene, Jared Ragland, Zachary Trautt, June W. Lau, Raymond Plante, Joshua Taillon, Adam Abel Creuziger, Chandler A. Becker, Joe Bennett, Niksa Blonder, Lisa Borsuk, Carelyn E. Campbell, Adam Friss, Lucas Hale, Michael Halter, Robert Hanisch, Gary R. Hardin, Lyle E. Levine, Samantha Maragh, Sierra Miller, Chris Muzny, Marcus William Newrock, John Perkins, Anne L. Plant, Bruce D. Ravel, David J. Ross, John Henry J. Scott, Christopher Szakal, Alessandro Tona, Peter Vallone
Instrumentation generates data faster and in higher quantity than ever before, and interlaboratory research is in historic demand domestically and internationally to stimulate economic innovation. Strategic mission needs of the NIST Material Measurement

Single-cell measurement of plasmid copy number and promoter activity

March 5, 2021
Bin Shao, Jayan Rammohan, Daniel Anderson, Nina Alperovich, David J. Ross, Christopher Voigt
We have developed a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of

Genetic circuit design automation

April 1, 2016
Alec Nielsen, Bryan Der, Jonghyeon Shin, Prashant Vaidyanathan, Douglas Densmore, Vanya M. Paralanov, Elizabeth Strychalski, David J. Ross, Christopher Voigt
Computation can be performed in living cells using DNA-encoded circuits that process sensory information and control biological functions. Their construction is time-intensive, requiring manual part assembly and balancing of regulator expression. We

Thermodynamics of the Hydrolysis Reactions of +--D-galactose 1-phosphate, sn-glycerol 3-phosphate, 4 nitrophenyl phosphate, phosphocreatine, and 3-phospho-D-glycerate

May 1, 2009
Robert N. Goldberg, Brian E. Lang, Catherine T. Lo, David J. Ross, Yadu D. Tewari
Microcalorimetry, high-performance liquid chromatography, and an enzymatic assay have been used to conduct a thermodynamic investigation of five phosphate hydrolysis reactions: α-D-galactose 1 phosphate(aq) + H2O(l) = D-galactose(aq) + orthophosphate(aq)

Multiplexed Detectorless Electrophoresis

December 15, 2008
David J. Ross, Jason G. Kralj
A new microfluidic electrophoresis device and technique is described that is designed specifically for multiplexed, high throughput separations. The device consists of an array of short (3 mm) capillaries connecting individual sample reservoirs to a common

Electrokinetic Sample Dilution and Concentration in Microfluidics

October 16, 2008
David J. Ross, K G. Olsen, Laurie E. Locascio
A new method is demonstrated which can be used to produce streams of variable, controlled analyte concentration. The method works by electrokinetically manipulating the analyte velocity so that it is different in different parts of a channel. Because of

Contactless Differential Conductivity Detection

October 12, 2008
Gordon A. Shaw, David J. Ross, Steven E. Fick, Wyatt N. Vreeland
We propose a new technique, contactless differential conductivity detection (CDCD,) to improve the detection limit of contactless conductivity detection for capillary and microchannel electrophoresis. By exploiting a 3-electrode differential configuration

Microfluidic DNA Analysis Systems for Forensic Applications

July 18, 2008
Michael Gaitan, Jayna J. Shah, Darwin Reyes-Hernandez, Pierre-Alain Auroux, Jon Geist, Laurie E. Locascio, Wyatt N. Vreeland, David J. Ross, Peter Vallone, Paul Smith, Nicole Morgan, Tom Pohida, John Kakareka, Annelise Barron
This report summarizes the NIST effort on microfluidic DNA analysis systems for forensic applications sponsored by the National Institute of Justice. Currently emerging microfluidics-based forensic systems are implemented in silica (glass) because the

Counter-Flow Gradient Electrofocusing

February 1, 2007
Jonathan G. Shackman, David J. Ross
Counter-flow gradient electrofocusing methods are methods whereby a combination of electrophoresis and a bulk solution counter-flow is used to accumulate or focus analytes at stationary points along a separation column. This review first describes the