Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Publications

Search Publications by David J. Ross

Search Title, Abstract, Conference, Citation, Keyword or Author
Displaying 1 - 25 of 50

The allosteric landscape of the lac repressor

May 21, 2021
Author(s)
Drew S. Tack, Peter Tonner, Abe Pressman, Nathanael David Olson, Eugenia Romantseva, Nina Y. Alperovich, Olga B. Vasilyeva, David Ross, Sasha F. Levy
Allostery is a fundamental biophysical mechanism where the activity of a biomolecule is regulated by the binding of a ligand. Despite playing a central role in many biological processes, a quantitative understanding of allostery is lacking. To

Single-cell measurement of plasmid copy number and promoter activity

March 5, 2021
Author(s)
Bin Shao, Jayan Rammohan, Daniel Anderson, Nina Y. Alperovich, David Ross, Christopher Voigt
We have developed a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of

Genetic circuit design automation

April 1, 2016
Author(s)
Alec Nielsen, Bryan Der, Jonghyeon Shin, Prashant Vaidyanathan, Douglas Densmore, Vanya M. Paralanov, Elizabeth A. Strychalski, David J. Ross, Christopher Voigt
Computation can be performed in living cells using DNA-encoded circuits that process sensory information and control biological functions. Their construction is time-intensive, requiring manual part assembly and balancing of regulator expression. We

Thermodynamics of the Hydrolysis Reactions of +--D-galactose 1-phosphate, sn-glycerol 3-phosphate, 4 nitrophenyl phosphate, phosphocreatine, and 3-phospho-D-glycerate

May 1, 2009
Author(s)
Robert N. Goldberg, Brian E. Lang, Catherine T. Lo, David J. Ross, Yadu D. Tewari
Microcalorimetry, high-performance liquid chromatography, and an enzymatic assay have been used to conduct a thermodynamic investigation of five phosphate hydrolysis reactions: α-D-galactose 1 phosphate(aq) + H2O(l) = D-galactose(aq) + orthophosphate(aq)

Multiplexed Detectorless Electrophoresis

December 15, 2008
Author(s)
David J. Ross, Jason G. Kralj
A new microfluidic electrophoresis device and technique is described that is designed specifically for multiplexed, high throughput separations. The device consists of an array of short (3 mm) capillaries connecting individual sample reservoirs to a common

Contactless Differential Conductivity Detection

October 12, 2008
Author(s)
Gordon A. Shaw, David J. Ross, Steven E. Fick, Wyatt N. Vreeland
We propose a new technique, contactless differential conductivity detection (CDCD,) to improve the detection limit of contactless conductivity detection for capillary and microchannel electrophoresis. By exploiting a 3-electrode differential configuration

Microfluidic DNA Analysis Systems for Forensic Applications

July 18, 2008
Author(s)
Michael Gaitan, Jayna J. Shah, Darwin R. Reyes-Hernandez, Pierre-Alain Auroux, Jon C. Geist, Laurie E. Locascio, Wyatt N. Vreeland, David J. Ross, Peter M. Vallone, Paul Smith, Nicole Morgan, Tom Pohida, John Kakareka, Annelise Barron
This report summarizes the NIST effort on microfluidic DNA analysis systems for forensic applications sponsored by the National Institute of Justice. Currently emerging microfluidics-based forensic systems are implemented in silica (glass) because the

Counter-Flow Gradient Electrofocusing

February 1, 2007
Author(s)
Jonathan G. Shackman, David J. Ross
Counter-flow gradient electrofocusing methods are methods whereby a combination of electrophoresis and a bulk solution counter-flow is used to accumulate or focus analytes at stationary points along a separation column. This review first describes the

Scanning Temperature Gradient Focusing

August 2, 2006
Author(s)
S J. Hoebel, K M. Balss, Barbara J. Jones, C Malliaris, Matt S. Munson, Wyatt N. Vreeland, David J. Ross
Temperature gradient focusing (TGF) is a recently developed technique for the simultaneous concentration and electrophoretic separation of ionic analytes in microfluidic channels. One drawback to TGF as it has previously been described is the limited peak