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Search Publications

NIST Authors in Bold

Displaying 26 - 50 of 1261

Proteomics approaches to ecoimmunology: new insights into wildlife immunity and disease

June 24, 2025
Author(s)
Amanda Vicente-Santos1, Natalia Herrera, Gabor Czirjak, Benjamin Neely, Daniel Becker
Understanding wildlife immune responses is crucial for assessing disease risks, environmental stress effects, and conservation challenges. Traditional ecoimmunology approaches rely on targeted assays, which, while informative, often provide a fragmented

Gene Copy Number Dictates Extracellular Vesicle Cargo

June 7, 2025
Author(s)
Sumeet Poudel, Zhiyong He, Jerilyn Izac, Lili Wang
Extracellular vesicles (EVs) are membrane-surrounded vesicles that carry heterogeneous cellular components, including proteins, nucleic acids, lipids, and metabolites. EVs' intravesicular and surface contents possess many biomarkers of physiological and

VerteBrain reveals novel neuronal and non-neuronal protein assemblies conserved across vertebrate brains

May 28, 2025
Author(s)
Vy Dang, Brittney Voigt, David Yang, Gabriel Hoogerbrugge, Muyoung Lee, Rachael Cox, Ophelia Papoulas, Claire McWhite, Raksha Pradeep, Janelle Leggere, Benjamin Neely, Ryan Gray, Edward Marcotte
Protein-protein interactions in the vertebrate brain underlie key processes such as neurotransmitter release, receptor activation, and signaling pathways that are essential for learning, memory, and cognition. Here, we systematically map protein-protein

Elongated particles in flow: commentary on small-angle scattering investigations

May 23, 2025
Author(s)
Guan-Rong Huang, Lionel Porcar, Ryan Murphy, Yuya Shinohara, Yangyang Wang, Jan-Michael Carrillo, Bobby G. Sumpter, Chi-Huan Tung, Lijie Ding, Changwoo Do, Wei-Ren Chen
This work thoroughly examines several analytical tools, each possessing a different level of mathematical intricacy, for the purpose of characterizing the orientation distribution function of elongated objects under flow. Our investigation places an

Top-Down Scoring of Spectral Fitness by Image Analysis for Protein Structure Validation

May 6, 2025
Author(s)
Benjamin Harding, Barry DeZonia, Rajat Garg, Ziling Hu, Frank Delaglio, Tim Grant, Chad Rienstra
Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique for protein structure determination, but traditional approaches require extensive manual assignment of hundreds to thousands of resonances. Here we present NMRFAM-BPHON, a novel "top

Certification of Standard Reference Material(R) 1989 Monodisperse Irregularly Shaped Epoxy-Based Particles (Nominal 100 mm, 150 mm, 220 mm)

May 2, 2025
Author(s)
Srivalli Telikepalli, Dean Ripple, Michael Carrier, Kristen Steffens, David Newton, Christopher Montgomery, Nicholas Ritchie, Anthony Asmar, Michael Halter
Standard Reference Material (SRM) 1989 is a NIST particle standard produced using photolithographic methods and delivers a certified value for particle size of an irregularly shaped particle. This SRM was developed to allow a more accurate monitoring of

A Call for Built-In Biosecurity Safeguards for Generative AI Tools

April 28, 2025
Author(s)
Mengdi Wang, Zaixi Zhang, Amrit Singh Bedi, Alvaro Velasquez, Stephanie Guerra, Sheng Lin-Gibson, Le Cong, Megan Blewett, Yuanhao Qu, Jian Ma, Eric Xing, George Church, Souradip Chakraborty
The rapid adoption of generative AI (GenAI) in biotechnology offers immense potential but also raises serious safety concerns. AI models for protein engineering, genome editing, and molecular synthesis can be misused to enhance viral virulence, design

Interlaboratory Assessment of Candidate Reference Materials for Lentiviral Vector Copy Number and Integration Site Measurements

April 21, 2025
Author(s)
Hua-Jun He, Zhiyong He, Steven Lund, Barbara Paugh, Jennifer McDaniel, Justin Zook, Sierra Miller, Samantha Maragh, Simona Patange, Mahir Mohiuddin, Alessandro Tona, John Elliott, Kenneth Cole, Sheng Lin-Gibson
Reference materials are essential for accurately measuring integrated lentiviral vector (LV) copy number (VCN) and integration sites for the safety and efficacy of lentivirus-based gene therapy. We conducted an interlaboratory study on NIST candidate

Recommended Nomenclature Convention for the NISTCHO Cell Line and Its Product Monoclonal Antibody, cNISTmAb.

April 11, 2025
Author(s)
Megan Cleveland, Ioannis Karageorgos, John Marino, Michael Tarlov, Katharina Yandrofski, Rebecca Zangmeister, Zvi Kelman
A Chinese hamster ovary (CHO) cell line expressing the same amino acid sequences of the heavy and light chains of the NIST monoclonal antibody, NISTmAb Reference Material 8671, was generated by MilliporeSigma to be developed by NIST as a reference material

Development and characterization of the NISTCHO reference cell line

March 18, 2025
Author(s)
Hussain Dahodwala, Irfan Hodzic, Alexei Slesarev, Ben Cutak, Alexander Kuzin, Rahul Lal, Jiajian Liu, James Mahon, Rajagopalan Lakshmi Narasimhan, Jaya Onuska, James Ravellette, Kelsey Reger, Sadie Sakurada, Floy Stewart, Trissa Borgschulte, Colette Cote, Kelvin Lee, Zvi Kelman, William Brad O'Dell, Britta Anderson
Well characterized reference materials enable successful collaborations within the scientific community by establishing common reagents for benchmarking studies and reducing the barriers to sharing materials and information. Here, we report the development

Measuring the size of oil droplets in a flow cytometer using Mie Resonances

January 21, 2025
Author(s)
Richard E. Cavicchi, Dean Ripple, Joshua Welsh, Jerilyn Izac, Alexander Peterson, Aaron Goldfain, Wyatt Vreeland
An emulsion of silicone oil droplets in aqueous buffer produces a distinctive series of peaks or resonances in the side scatter histogram in a flow cytometer. As many as 12 peaks are observed in the violet-side scatter channel at 405 nm, with half that

Interlaboratory Measurement of Adeno-Associated Virus: Comparative Quantification of Full and Empty Capsids

December 26, 2024
Author(s)
Sean Lehman, Wyatt Vreeland, Jerome Jacques, Sharee Adams-Hall, Shreya Ahuja, Adnan Arnaout, Hunter Balduf, Ivan Budyak, Thomas Cleveland, Dennis Delgado, James Deng, Brandon Doyle, David Duewer, Carsten Elger, Jeffrey Fagan, Tim Guo, Jorge Haller, Luisa Hilgenfeld, Van Hoang, Allison Huldin, Matthew Hyatt, Sambit Kar, Sandeep Kedia, Bashkim Kokona, Amy Liu, Li Ma, Diane McCarthy, He Meng, Easton Noble, Veronika Oettle, Andrew Pla, Thomas Powers, Dean Ripple, Herbert Runnels, Raphael Ruppert, Florian Semmelmann, Christopher Sims, Saurabh Singh, Ronald Toth, Austin Vogt, Sabine Wenzel, Neal Whitaker, Zhiwen Yang, Brandon Zhuang
Recombinant adeno-associated virus (AAV) is one of the main viral vector-based gene therapy platforms. AAV is a virus consisting of a ≈25 nm diameter capsid with a ≈4.7 kb cargo capacity. The safety and efficacy depend on the correct encapsidation of the

The Application of Digital PCR as a Reference Measurement Procedure to Support the Accuracy of Quality Assurance for Infectious Disease Molecular Diagnostic Testing

December 26, 2024
Author(s)
Samreen Falak, Denise O'Sullivan, Megan Cleveland, Simon Cowen, Eloise Busby, Alison Devonshire, Esmeralda Valiente, Gerwyn Jones, Martin Kammel, Mojca Milavec, Laura Vierbaum, Ingo Schellenberg, Heinz Zeichhardt, Andreas Kummrow, Peter Vallone, Rainer Macdonald, Jim Huggett
Background Nucleic Acid Amplification Tests (NAATs) are used to diagnose many infectious diseases. They are typically sensitive and specific and can be quickly developed and adapted. It is more challenging to develop standards to ensure NAATs perform

Enabling Global Image Data Sharing in the Life Sciences

December 23, 2024
Author(s)
Anne L. Plant, Peter Bajcsy
Public, reference data are one of the most important foundational resources in the modern life and biomedical sciences. Over the last 40 years, the public release and availability of genomic and macromolecule structural databases accelerated discovery

Protocol for CRISPR-Cas9 genome editing of a swine cell line via electroporation

December 20, 2024
Author(s)
Patricia Kiesler, Samantha Maragh, Stella Lee, Alexis Norris, Mayumi Miller, Adam Moyer
Genome editing technology is being used in animals for a variety of purposes, including improvement of animal and public health outcomes. Characterization of genome editing reagents and anticipated genomic alterations is an essential step toward the

Advancing Cell-Free Manufacturing: Challenges in Scale-up and Automation Workshop Report

November 20, 2024
Author(s)
Eugenia Romantseva, Fernanda Piorino Macruz de Oliveira, Chad Sundberg, G. Sitta Sittampalam, Elizabeth Strychalski
The workshop on Advancing Cell-free Manufacturing: Challenges in Scale-up and Automation sought to identify and prioritize actionable steps towards more reproducible, scalable, and automated cell-free systems for applications in bioengineering and

Multiplexed SERS Detection of Serum Cardiac Biomarkers Using Plasmonic Metasurfaces

October 15, 2024
Author(s)
Peng Zheng, Lintong Wu, Piyush Raj, Jeong Hee Kim, Santosh Paidi, Stephen Semancik, Ishan Barman
Surface-enhanced Raman spectroscopy (SERS) possesses exquisite molecular-specific properties with single-molecule sensitivity. Yet, translation of SERS into a quantitative analysis technique remains elusive owing to considerable fluctuation of the SERS

Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards

October 3, 2024
Author(s)
Tracey Schock, Christina Jones, Federico Torta, Nils Hoffmann, Bo Burla, Irina Alecu, Makoto Arita, Takeshi Bamba, Steffany A.L. Bennett, Justine BERTRAND-MICHEL, Britta Brugger, Monica Molina, Maria Dolores Camacho Munoz, Antonio Checa, Michael Chen, Michelle Cinel, Benoit COLSCH, Cristina Coman, Bebiana Da Costa Sousa, Alex Dickens, Maria Maria, Finnur Freyr Eiriksson, Hector Gallart-Ayala, Mohan Ghorasaini, Martin Giera, Xue Guan, Mark Haid, Thomas Hankemeier, Amy Harms, Marcus Horing, Michal Holcapek, Thorsten Hornemann, Chunxiu Hu, Andreas Hulsmeier, Julijana Ivanisevic, Harald Kofeler, Sin Man Lam, Jong Cheol Lee, Gerhard Liebisch, Andrea F Lopez-Clavijo, Malena Manzi, Peter Meikle, Maria Monge, Sneha Muralidharan, Anna Nicolaou, Valerie O'Donnell, Matej Oresic, Arvind Ramanathan, Daisuke Saigusa, Heidi Schwartz-Zimmermann, Guanghou Shui, Masatomo Takahashi, Margret Thorsteinsdottir, Anthony Tournadre, Hiroshi Tsugawa, Victoria Tyrrell, Grace van der Gugten, Michael Wakelam, Craig Wheelock, Denise Wolrab, Guowang Xu, John Bowden, Kim Ekroos, Robert Ahrends, Markus Wenk
In this community effort, we compared measurements between 34 laboratories from 19 countries, utilizing mixtures of authentic synthetic standards, to quantify by mass spectrometry four ceramide species in the NIST human blood plasma Standard Reference
Displaying 26 - 50 of 1261
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