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Health

Pharmaceuticals

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Displaying 1 - 25 of 122

Advances in Yeast Synthetic Biology for Human GPCR Biology and Pharmacology

July 29, 2024
Author(s)
Geoffrey Taghon, Nicholas Kapolka, Daniel Isom
G protein-coupled receptors (GPCRs) are the largest family of transmembrane receptors in humans. Over 800 GPCRs regulate the (patho)biology of every organ, tissue, and cell type. Consequently, GPCRs are the most prominent therapeutic targets in medicine

Recommendations on fit-for-purpose criteria to establish a quality management for microphysiological systems (MPS) and for monitoring of their reproducibility

May 14, 2024
Author(s)
David Pamies, Jason Ekert, Marie-Gabrielle Zurich, Olivier Frey, Sophie Werner, MONICA PIERGIOVANNI, Benjamin Freedman, Adrian Keong, Darwin Reyes-Hernandez, Hendrik Erfurth, Peter Loskill, Pelin Candarlioglu, Shan Wang, Thomas Hartung, Sandra Coecke, Glyn Stacey, Marcel Leist
Cell culture technology has seen great innovations and progress in the 21st centuryClassical single-cell and monolayer models have been replaced by more complex 3D models (e.g., reaggregates, spheroids, organoids) to improve the predictivity of in vitro

From animal testing to in vitro systems: advancing standardization in microphysiological systems

February 15, 2024
Author(s)
Darwin Reyes-Hernandez, Mandy Esch, Lorna Ewart, Rohollah Nasiri, Anna Herland, Carolina Lucchesi, Kyung Sung, MONICA PIERGIOVANNI, Jelena Vukasinovic, James Shoemaker, Nureddin Ashammakhi
Limitations with cell cultures and experimental animal-based studies have had the scientific and industrial communities searching for new approaches that can provide reliable human models for applications such as drug development, toxicological assessment

Heart-on-a-Chip Systems: Disease Modeling and Drug Screening Applications

February 14, 2024
Author(s)
Derrick Butler, Darwin Reyes-Hernandez
Cardiovascular disease (CVD) is the leading cause of death worldwide, casting a substantial economic footprint and burdening the global healthcare system. Historically, pre-clinical CVD modeling and therapeutic screening has been performed using animal

Single-Detector Double-Beam Modulation for High-Sensitivity Infrared Spectroscopy

October 25, 2023
Author(s)
Seongmin Kim, Yow-Ren Chang, Young Jong Lee
Balanced detection based on double beams is widely used to reduce common-mode noises, such as laser intensity fluctuation and irregular wavelength scanning, in absorption spectroscopy. However, employing an additional detector can increase the total system

RECENT DEVELOPMENTS IN ONTOLOGY STANDARDS AND THEIR APPLICABILITY TO BIOMANUFACTURING

August 21, 2023
Author(s)
Milos Drobnjakovic, Boonserm Kulvatunyou, Vijay Srinivasan, Simon P. Frechette
ISO and IEC have jointly initiated, and recently issued, a series of standards (the ISO/IEC 21838 series) for top-level ontologies. These standards have been used by industrial consortia to develop and disseminate standards for mid-level ontologies to ease

Backbone NMR assignment of the yeast produced Fab fragment of the NISTmAb reference antibody

March 1, 2023
Author(s)
Tsega Solomon, Kinlin Chao, Genevieve Gingras, Yves Aubin, William Brad O'Dell, John Marino, Robert Brinson
The monoclonal antibody (mAb) protein class has become a primary therapeutic platform for the production of new life saving drug products. MAbs are comprised of two domains: the antigen-binding fragment (Fab) and crystallizable fragment (Fc). Despite the

An Interlaboratory Study of Potential Visible Protein-Like Particle Standards

November 1, 2022
Author(s)
Srivalli Telikepalli, Michael J. Carrier, Dean C. Ripple
Visible protein-like particle standards can improve visual inspection and/or appearance testing practices used in the biotechnology industry. It will improve assay performance resulting in better alignment and more standardized training among different

Primary standardization of 212Pb activity by liquid scintillation counting

September 22, 2022
Author(s)
Denis E. Bergeron, Jeffrey T. Cessna, Ryan P. Fitzgerald, Lizbeth Laureano-Perez, Leticia Pibida, Brian E. Zimmerman
An activity standard for 212Pb in equilibrium with its progeny was realized, based on triple-to-double coincidence ratio (TDCR) liquid scintillation (LS) counting. A Monte Carlo-based approach to estimating uncertainties due to nuclear decay data

Distribution of Average Aggregate Density from Stir-stressed NISTmAb Protein

March 19, 2022
Author(s)
Richard E. Cavicchi, Laura Philips, Fook Cheong, David Ruffner, Priya Kasimbeg, Wyatt N. Vreeland
Quantifying the heterogeneous nature of protein aggregates is important to understanding the impact aggregates may have on the performance of antibody therapeutics. The spatially averaged density p of aggregates, defined as the total mass, including water

HDX-MS and MD simulations provide evidence for stabilization of the IgG1- FcgRIa (CD64a) immune complex through intermolecular glycoprotein bonds

December 8, 2021
Author(s)
Kyle Anderson, Kerry Scott, Christina Bergonzo, Ioannis Karageorgos, Elyssia Gallagher, Venkata Tayi, Michael Butler, Jeffrey W. Hudgens
Previous reports present different models for the stabilization of the Fc—FcγRI immune complex. Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of

An assessment of US microbiome research

September 23, 2021
Author(s)
Scott Jackson
Genome-enabled technologies have supported a dramatic increase in our ability to study microbial communities in environments and hosts. Taking stock of previously funded microbiome research can help to identify common themes, under-represented areas and

Comprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb and Their Use for Developing a Mass Spectral Library

June 22, 2021
Author(s)
Qian Dong, Xinjian Yan, Yuxue Liang, Sanford Markey, Sergey L. Sheetlin, Concepcion Remoroza, William E. Wallace, Stephen Stein
This work presents methods for identifying and then creating a mass spectral library for disulfide-linked peptides originating from the NISTmAb. Analysis involved both partially- reduced and non-reduced proteins under neutral and weakly basic conditions

Measurement of 227 Ac Impurity in 225 Ac using Decay Energy Spectroscopy

March 20, 2021
Author(s)
A. D. Tollefson, C. M. Smith, M. H. Carpenter, M. P. Croce, M. E. Fassbender, K. D. John, K. E. Koehler, L. M. Lilley, E. M. O'Brien, Daniel Schmidt, B. W. Stein, Joel Ullom, M. D. Yoho, D. J. Mercer
225Ac is a valuable medical isotope for targeted alpha therapy, but 227Ac is an undesirable byproduct of an accelerator-based synthesis method under investigation. Sufficient detector sensitivity is critical for quantifying the trace impurity of 227Ac

Design of a novel, native-like secreted form of the hepatitis C virus E1E2 heterodimer

January 11, 2021
Author(s)
Johnathan D. Guest, Ruixue Wang, Khadija Elkholy, Andrezza Chagas, Kinlin L. Chao, Thomas Cleveland, Young C. Kim, Zhen-Yong Keck, Alexander Marin, Roy Mariuzza, Alexander K. Andrianov, Eric A. Toth, Steven Foung, Brian Pierce, Thomas R. Fuerst
Hepatitis C virus (HCV) is a major worldwide health burden, and a preventive vaccine is needed for global control or eradication of this virus. A substantial hurdle to an effective HCV vaccine is the high variability of the virus, leading to immune escape
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