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Preparation of Calibration Solutions



Lane C. Sander, Michele M. Schantz


Calibration solutions provide the basis for quantitation for most instrumental techniques. The instrumental responses for analytes in calibrants of known composition are compared with corresponding responses in samples to provide quantitation of the constituents. Calibration solutions are used for external standard, internal standard, and standard addition approaches to quantitation. Calibrants can be prepared gravimetrically using reference standards or reference materials of known purity; however, volumetric or other fraction basis is also possible. Calibrants are usually prepared with pure solvents, but the use of natural or other complex matrix materials such as biological fluids is also possible. For such cases, the materials must be well characterized to assure the absence of matrix interferences. This presentation will demonstrate gravimetric preparation of solution calibrants with solid and liquid constituents. Details are described for special requirements employed with the certification of Standard Reference Materials.
Journal of Research (NIST JRES) -


calibration, gravimetry, balance, calibrant, weighing liquids, weighing solids


Sander, L. and Schantz, M. (2017), Preparation of Calibration Solutions, Journal of Research (NIST JRES), National Institute of Standards and Technology, Gaithersburg, MD, [online], (Accessed May 29, 2024)


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Created January 12, 2017, Updated January 27, 2020