Interlaboratory Comparison with a Reference Measurement Procedure (RMP) for Determining 24,25-Dihydroxvitamin D3 in Human Serum using Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS)

Published

April 28, 2026

Author(s)

Stephen Wise, Ekaterina Mineva, Christine Pfeiffer, Hubert Vesper, Etienne Cavalier, Stephenie Peters, Karen Galvin, Kevin Cashman, Andrew Norbert Hoofnagle, Hsuan-Chieh (Joyce) Liao, Emma Williams, Laura Briggs, Adam Kuszak, Grace Hahm, Johanna Camara

Abstract

An interlaboratory comparison study was conducted among five laboratories for determination of 24,25-dihydroxyvitamin D3 [24,25(OH)2D3] in human serum using liquid chromatography - tandem mass spectrometry (LC-MS/MS) methods. The Centers for Disease Control and Prevention (CDC), Imperial College Healthcare NHS Trust, University College Cork, University of Liège, and University of Washington analyzed 50 single-donor samples and two new Standard Reference Materials (SRMs®). The results from each laboratory were compared with target values assigned by the National Institute of Standards and Technology (NIST) using a reference measurement procedure (RMP) and evaluated using Ordinary Deming linear regression and Bland-Altman analysis. Three of the five laboratory methods provided results that were in good agreement with the NIST RMP results, i.e., linear regression slopes of 0.972 to 1.003 and Bland-Altman mean bias of -0.092 nmol/L to 0.035 nmol/L. Two laboratories demonstrated a significant positive bias with linear regression slopes of 1.158 and 1.214 and Bland-Altman mean bias of 0.162 nmol/L and 0.708 nmol/L. The CDC method is currently used to assign "information only" values for 24,25(OH)2D3 in quarterly distributions of the Vitamin D External Quality Assessment Scheme (DEQAS). Based on the agreement of the CDC method with the NIST RMP, the CDC-assigned DEQAS values for 24,25(OH)2D3 may offer a better accuracy basis than the current participant consensus values.

Citation

Analytical and Bioanalytical Chemistry

Pub Type

Journals

Download Paper

https://doi.org/10.1007/s00216-026-06480-8

Local Download

Keywords

Certified Reference Materials, Standard Reference Materials (SRMs), 24, 25-Dihydroxyvitamin D3, 25-hydroxyvitamin D3, 25-hydroxyvitamin D2, Vitamin D External Quality Assessment Scheme (DEQAS)

Citation

Wise, S. , Mineva, E. , Pfeiffer, C. , Vesper, H. , Cavalier, E. , Peters, S. , Galvin, K. , Cashman, K. , Hoofnagle, A. , Liao, H. , Williams, E. , Briggs, L. , Kuszak, A. , Hahm, G. and Camara, J. (2026), Interlaboratory Comparison with a Reference Measurement Procedure (RMP) for Determining 24,25-Dihydroxvitamin D3 in Human Serum using Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS), Analytical and Bioanalytical Chemistry, [online], https://doi.org/10.1007/s00216-026-06480-8, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=961147 (Accessed April 29, 2026)

Additional citation formats