Standard Reference Material (SRM) 2941 is a cuvette-shaped, uranyl-ion-doped glass, recommended for use for relative spectral correction of emission and day-to-day performance validation of fluorescence spectrometers. Properties of this standard that influence its effective use or contribute to the uncertainty in its certified emission spectrum have been explored here. These properties include its photostability, absorbance, dissolution rate in water, anisotropy, temperature coefficient of fluorescence intensity and fluorescence lifetimes. The expanded uncertainties in the certified spectrum are about 4 % around the peak maximum at 526 nm, using an excitation wavelength of 427 nm. The SRM also exhibits a strong resistance to photodegradation, with no measurable decrease in fluorescence intensity even after 8 hours of laser irradiation.
Citation: Journal of Luminescence
Pub Type: Journals
biosystems and helath, calibration, fluorescence, instrument qualification, method validation, spectral correction, SRM