Skip to main content

NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Kirsten Parratt (Fed)

Biomedical Engineer

OVERVIEW

Kirsten Parratt, PhD is a Biomedical Engineer in the Biomaterials Group.

Microbial cell characterization work: Kirsten leads flow cytometry efforts related to the quantitation and characterization of microbial cells in support of Rapid Microbial Testing Methods for advanced therapy products and whole cell microbial reference material development (including the new Whole Gut Microbiome RM). 

Data and metadata work: Kirsten co-leads projects on data standards development (ASTM WK83732) and research data management (electronic lab notebook, data repository) for Microbial Metrology. She is also a member of the ISAC Data Committee with a focus on Standards and Metadata.

Previous work: Kirsten was a NIST-NRC Postdoctoral Research Associate mentored by Nancy J. Lin. The goal of her NRC work was to develop quantitative measurements of adherent microbial communities and biofilm-material interactions. Her graduate dissertation research under Krish Roy focused on using flow cytometry as a high-throughput platform to assay microfabricated biomaterials for stem cell differentiation studies and detection applications.

PUBLICATIONS

  • Servetas, SL., Parratt, KH., Brinkman, NE., Shanks, OC., Smith, T., Mattson, PJ., Lin, NJ. “Standards to support an enduring capability in wastewater surveillance for public health: Where are we?” Case Studies in Chemical and Environmental Engineering (2022). https://www.sciencedirect.com/science/article/pii/S266601642200069X
  • Inwood, SL., Tian, L., Parratt, K., Maragh, S., Wang, L. “Evaluation protocol for CRISPR/Cas9-mediated CD19 knockout GM24385 cells by flow cytometry and Sanger sequencing” Biotechniques (2022). https://www.future-science.com/doi/full/10.2144/btn-2022-0015
  • Lin, NJ., Servetas, SL., Jackson, SA., Lippa, KA., Parratt, K., Mattson, P., Beahn, C., Mattioli, M., Gutierrez, S., Focazio, M., Smith, T., Storella, P., Wright, S. “Report on the DHS/NIST Workshop on Standards for an Enduring Capability in Wastewater Surveillance for Public Health” (2022). https://nvlpubs.nist.gov/nistpubs/SpecialPublications/NIST.SP.1279.pdf
  • Lin, NJ., Jackson, SA., Servetas, S., Parratt, K., Dunkers, J., Eskandari, T., Lin-Gibson, S. “Report from the NIST Workshop: Launch of the Rapid Microbial Testing Methods Consortium” (2022). https://nvlpubs.nist.gov/nistpubs/SpecialPublications/NIST.SP.1272.pdf
  • Parratt, K.; Jeong, J.; Qiu, P.; Roy, K. 3D Material Cytometry (3DMaC): A Very High-replicate, High-throughput Analytical Method using Microfabricated, Shape-specific, Cell-material Niches. Lab on a Chip, 2017, 17, 2861-2872.
  • Parratt, K.; Smerchansky, M.; Stiggers, Q.; Roy, K. Effect of Hydrogel Material Composition on hBMSC Differentiation into Zone-specific Neo-cartilage: Engineering Human Articular Cartilage with Spatially Varying Properties. Journal of Materials Chemistry B, 2017, 5, 6237-6248.
  • Dwarshuis, N.; Parratt, K.; Santiago-Miranda, A.; Roy, K. Cells as Advanced Therapeutics: State-of-the-art, Challenges, and Opportunities in Large Scale Biomanufacturing of High-Quality Cells for Adoptive Immunotherapies. Advanced Drug Delivery Reviews, 2017, 114, 222-239.

Publications

NIST 2025 Rapid Microbial Testing Methods Workshop Report

Author(s)
Jason Kralj, Kirsten Parratt, Tyler Laird, Stephanie Servetas, Nancy Lin, Dawn Henke, Scott Jackson
In 2020, the National Institute of Standards and Technology (NIST)-led Rapid Microbial Testing Methods (RMTM) Consortium was established to develop standards

NIST 2024 Rapid Microbial Testing Methods Workshop Report

Author(s)
Jason Kralj, Nancy Lin, Kirsten Parratt, Stephanie Servetas, Dawn Henke, Scott Jackson
The NIST-led Rapid Microbial Testing Methods (RMTM) Consortium launched in 2020 to develop standards and measurement-based solutions to advance the use of RMTMs

Data and Software Publications

Measurement Quality Metrics to Improve Absolute Microbial Cell Counting

Author(s)
Kirsten Parratt, David Newton, Joy Dunkers, Jennifer Dootz, Monique Hunter, Alshae' Logan-Jackson, Laura Pierce, Sumona Sarkar, Nancy J Lin
This repository contains the raw data and analysis scripts supporting the associated publication which introduces a framework to help researchers select fit-for-purpose microbial cell counting methods

Characterization of Human Fecal Material (NIST RM 8048) from Omnivore and Vegetarian Donors

Author(s)
Scott A Jackson, Stephanie L Servetas, Samuel P Forry, Monique E Hunter, Tracey B Schock, W Clay Davis, Amanda L Bayless, Fabio Casu, Debra L Ellisor, Aaron A Urbas, Sandra M Da Silva, Kirsten Parratt, Krissie Gierz, Hari Iyer
This repository holds the raw data, analysis code, and results generated in the characterization of NIST Reference Material RM 8048 - Human Fecal Material (RM 8048) from Omnivore and Vegetarian Donors
Created May 7, 2019, Updated March 19, 2024
Was this page helpful?