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John P. Marino (Fed)

Group Leader

My research is focused on developing NMR and other biophysical measurements to accurately and precisely define the structure, stability and dynamics of protein and RNA structural folds, ligand complexes and biomolecular interactions at a molecular level.  In addition to enabling fundamental insights into biomolecular structure and function, our work aims to provide a validated measurement infrastructure that supports biopharmaceutical development and regulation.  Our most recent work has focused on the development of NMR techniques for accurate and precise characterization of higher-order-structure (HOS) in biotherapeutics, in particular monoclonal antibodies, which are currently sought by industry for establishing consistency in drug manufacturing, detecting process-related drug-product variations and comparing biosimilars to innovator reference products.  We are developing and demonstrating high-resolution NMR methods as simple, robust spectroscopic approaches for obtaining structural 'fingerprints' of the bioactive form(s) of protein therapeutics at atomic resolution in solution. 


Department of Commerce Silver Medal Award  2007, 2016 and 2021

Hillebrand Prize 2021


Patents (2018-Present)

Amino Acid-Specific Binder And Selectively Identifying An Amino Acid

NIST Inventors
John P. Marino and Zvi Kelman
N-terminal amino acid binding (NAAB) reagents are a tool for parallel, high-throughput proteomics. Afluorescently-labeled NAAB allows immobilized peptides to be identified by their N-terminal residues using single-molecule fluorescent microscopy, which enables novel proteomic analysis, like

Amino Acid-specific Binder and Selectively Identifying an Amino Acid

NIST Inventors
Zvi Kelman and John P. Marino
One of the central challenges in the development of single-molecule protein sequencing technologies is achieving high- fidelity, sequential recognition and detection of specific amino acids that comprise the peptide sequence. The N-End Rule Pathway adaptor protein ClpS, natively recognizes N
Created June 18, 2019, Updated December 8, 2022