Levy, D.
and Briggman, K.
(2007),
Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes, Biophysical Journal, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=841051 (Accessed April 28, 2026)
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes
Published
Author(s)
,
Abstract
The interactions between cholesterol and saturated phospholipids in hybrid bilayer membranes (HBMs) were investigated using the interface-sensitive technique of vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to order/disorder transitions of the lipid acyl chains was used to determine the main gel to liquid crystal phase transition temperature, Tm, for HBMs of binary cholesterol/phospholipid mixtures on octadecanethiolate self-assembled monolayers. The phase transition temperature and the breadth of the transition were shown to increase with cholesterol content, and the phase boundaries observed in the cholesterol/phospholipid HBMs were comparable to the published phase diagrams of binary cholesterol/phospholipid vesicles. A thermodynamic assessment of the cooperative units of theHBMphase transitions revealed the presence of<10nmdiameter domains that were independent of the cholesterol composition.Citation
Biophysical Journal
Volume
23
Pub Type
Journals
Download Paper
Keywords
cholesterol-lipid phase diagrams, hybrid bilayer membranes, SFG, supported bilayer membrane mimics, vibrational sum frequency
Citation
Issues
If you have any questions about this publication or are having problems accessing it, please contact [email protected].
Created May 24, 2007, Updated October 12, 2021