Edward Kwee (Fed)

Research Interests

Edward is focused on developing research and standards solutions to support advanced measurements of gene delivery systems. His interests are in improving the reproducibility of the functional evaluation of gene delivery systems through cell based assays. He helps coordinate gene delivery system program efforts in the Biosystems and Biomaterials Division. Please visit the program page for more information. 

Edward participates in standards development for gene delivery systems through ISO Technical Committee 276 (TC 276) on Biotechnology, Subcommittee 1 on Analytical Methods (SC 1), and Working Group 1 on Gene Delivery Systems (WG 1). Edward serves as a project lead on multiple standards under developement and serves as a lead for the United States Technical Advisory group to ISO TC 276/SC 1/WG 1. Please visit the US Technical Advisory Group for ISO TC 276 page for more information and how to join. 

He is also developing novel measurement methods to characterize gene delivery particles by light scattering and correlating those measurements with functional assays. Please visit the project page for more information. 

Edward co-leads efforts in Working Group 4: Gene Delivery Systems in the NIST Flow Cytometry Standards consortium. Activities include developing protocols and evaluating gene delivery system materials in a physical titer interlaboratory study. Please visit the consortium page for more information. 

Postdocoral Research Opportunities

NIST offers two year postdoctoral research opporunities through the NRC Research Associateship Program. The program is open to US Citizens only, offers competitive salary and benefits, with research proposal and application deadlines annually on February 1 and August 1. Research proposal topics include, but are not limited to:

• Advancing Cell-Based Viral Infectivity Assays: Enhancing Reproducibility and Automation

Contact Edward and visit the NIST-NRC Postdoc webpage for more information.

Research Topics

• Quantitative assessment of viral vector infectivity (lentivirus, pseudovirus, adenovirus) dynamics and heterogeneity using cell based assays and live cell imaging
• Viral vector reference material development and characterization
• Measurement of proliferative and cytotoxic heterogeneity in chimeric antigen receptor T (CAR-T) cells using single cell, live cell imaging and machine learning models.
• Quantitative phase imaging (QPI) reference materials to enable reproducible and comparable QPI measurements between different experiments and laboratories

Past Publications

1. Kwee, E. et al. “Quantifying Proliferative and Surface Marker Heterogeneity in Colony Founding Connective Tissue Progenitors and Their Progeny Using Time-Lapse Microscopy” Journal of Tissue Engineering and Regenerative Medicine. (2019) 13(2):203-216.
2. Piuzzi, NS, Mantripragada, VP, Kwee, E, et al. “Bone Marrow-Derived Cellular Therapies in Orthopaedics: Part II: Recommendations for Reporting the Quality of Bone Marrow-Derived Cell Populations.” JBJS Reviews. (2018);6(11):e5.
3. Kwee, E, et al. "Integrated Colony Imaging, Analysis, and Selection Device for Regenerative Medicine." SLAS Technology. 22.2 (2017): 217-223.
4. Powell, K., Kwee, E., et al.. "Variability in subjective review of umbilical cord blood colony forming unit assay." Cytometry Part B: Clinical Cytometry, 2016. 90(6): 517-524.
5. Kwee, E., Powell, K., and Muschler, G. Characterization of connective tissue progenitors through phase contrast and multicolor fluorescence time-lapse microscopy ", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280O, 2015.

Sigma Xi Most Outstanding Biology Poster at NIST Post-doc Poster Presentation, 2018

BioImage Informatics Travel Award, 2017

National Research Council Postdoctoral Research Associateship, 2016

AAAS/Science Program for Excellence in Science, 2015

Alpha Epsilon: Honor Society of Biological Engineering, 2010

Cornell Engineering Co-op of the Year, 2009