Preparation of Calibration Solutions

Published

January 12, 2017

Author(s)

Lane C. Sander, Michele M. Schantz

Abstract

Calibration solutions provide the basis for quantitation for most instrumental techniques. The instrumental responses for analytes in calibrants of known composition are compared with corresponding responses in samples to provide quantitation of the constituents. Calibration solutions are used for external standard, internal standard, and standard addition approaches to quantitation. Calibrants can be prepared gravimetrically using reference standards or reference materials of known purity; however, volumetric or other fraction basis is also possible. Calibrants are usually prepared with pure solvents, but the use of natural or other complex matrix materials such as biological fluids is also possible. For such cases, the materials must be well characterized to assure the absence of matrix interferences. This presentation will demonstrate gravimetric preparation of solution calibrants with solid and liquid constituents. Details are described for special requirements employed with the certification of Standard Reference Materials.

Citation

Journal of Research (NIST JRES) -

NIST Pub Series

Journal of Research (NIST JRES)

Pub Type

NIST Pubs

Download Paper

https://doi.org/10.6028/jres.122.009

Keywords

calibration, gravimetry, balance, calibrant, weighing liquids, weighing solids

Citation

Sander, L. and Schantz, M. (2017), Preparation of Calibration Solutions, Journal of Research (NIST JRES), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/jres.122.009 (Accessed April 23, 2024)