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Pulsed-field-gradient NMR studies of nanoclusters and macromolecular crowding

Liquid-state NMR provides a way to obtain chemical signatures via the "chemical shift" spectrum. Pulsed-field-gradient (PFG) NMR measures spectrally selective diffusion coefficients, enabling one to obtain more than one diffusion coefficient from a single measurement.

Using PFG NMR, we can obtain detailed information on macromolecular size as well as deduce and quantify the presence of macromolecular complexes or clusters [1]. Two examples are discussed: clusters in lysozyme protein solutions [2], and dynamics in a polymer-polysucrose model system for macromolecular crowding [3].

[1] Suliman Barhoum, Swomitra Palit, Anand Yethiraj, "Diffusion NMR studies of macromolecular complex formation, crowding and confinement in soft materials", Progress in Nuclear Magnetic Resonance Spectroscopy, 94-95, 1-10 (2016).
[2] Suliman Barhoum, Anand Yethiraj, "NMR Detection of an Equilibrium Phase Consisting of Monomers and Clusters in Concentrated Lysozyme Solutions ", J. Phys. Chem. B 114, 17062-17067 (2010).
[3] Swomitra Palit, Lilin He, William A. Hamilton, Arun Yethiraj, Anand Yethiraj, "Combining diffusion NMR and SANS enables precise measurements of polymer chain compression in a crowded environment", Phys. Rev. Lett, 118, 097801 (2017).

Anand Yethiraj (Newfoundland and Labrador's University, Canada)

Created January 16, 2020