Skip to main content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Pulsed-field-gradient NMR studies of nanoclusters and macromolecular crowding

Liquid-state NMR provides a way to obtain chemical signatures via the "chemical shift" spectrum. Pulsed-field-gradient (PFG) NMR measures spectrally selective diffusion coefficients, enabling one to obtain more than one diffusion coefficient from a single measurement.

Using PFG NMR, we can obtain detailed information on macromolecular size as well as deduce and quantify the presence of macromolecular complexes or clusters [1]. Two examples are discussed: clusters in lysozyme protein solutions [2], and dynamics in a polymer-polysucrose model system for macromolecular crowding [3].

[1] Suliman Barhoum, Swomitra Palit, Anand Yethiraj, "Diffusion NMR studies of macromolecular complex formation, crowding and confinement in soft materials", Progress in Nuclear Magnetic Resonance Spectroscopy, 94-95, 1-10 (2016).
[2] Suliman Barhoum, Anand Yethiraj, "NMR Detection of an Equilibrium Phase Consisting of Monomers and Clusters in Concentrated Lysozyme Solutions ", J. Phys. Chem. B 114, 17062-17067 (2010).
[3] Swomitra Palit, Lilin He, William A. Hamilton, Arun Yethiraj, Anand Yethiraj, "Combining diffusion NMR and SANS enables precise measurements of polymer chain compression in a crowded environment", Phys. Rev. Lett, 118, 097801 (2017).

Anand Yethiraj (Newfoundland and Labrador's University, Canada)

Created January 16, 2020