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VIDEOS

Phase Contrast and Fluorescence Images of an iPSC Colony in Time

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Cells (from the Allen Institute for Cell Science) are expressing a chromosomal marker, H2B, which is a histone protein. Cells that are dividing can be distinguished by the appearance of their fluorescence. By imaging live cells over long times we can quantify the number of cells, the number of dividing cells, and the time between cell divisions, and we can study how colonies change over time, and how location within a colony may influence cell division. We also use the fluorescence data in training neural networks so that phase contrast images of non-labeled cells can be quantified.

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January 25, 2023