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Volumetric Transfer of Liquids

Published

Author(s)

Lane C. Sander

Abstract

Although volumetric measurements are usually less accurate than gravimetric based measurements, volumetric measurements are still in common use due to the convenience, and often higher throughput, that may be afforded by automatic pipettes. Analysts should be aware of the performance limitations that can be expected for methods that rely on volumetric measurements, and precautions that must be observed to achieve the best results. This presentation highlights the use of different types of automatic pipettes and demonstrates pipetting operations for aqueous and organic liquids. Topics include pipette tip selection, positive displacement and air displacement pipettes, operation of mechanical and electronic pipettes, and performance expectations.
Citation
Journal of Research (NIST JRES) -
Volume
122

Keywords

pipette, volumetric transfer, quantitation, positive displacement pipette, air displacement pipette

Citation

Sander, L. (2017), Volumetric Transfer of Liquids, Journal of Research (NIST JRES), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/jres.122.002 (Accessed July 16, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created January 12, 2017, Updated January 27, 2020