Tunable glass reference materials for quantitative backscattered electron imaging of mineralized tissues
Sara E. Campbell, Roy H. Geiss, Steve A. Feller, Virginia L. Ferguson
Backscatter electron (BSE) microscopy provides graylevel contrast resulting from variations in atomic composition. Through the use of standards, quantitative BSE imaging can be used to measure the mineral content of mineralized tissues such as bone and teeth. We developed novel tunable standards that can be adjusted for analysis of an individual tissue or a wide range of tissues with variable mineral content. As an alternative to metallic standards, these amorphous standards maintain long-term stability and possess no long-range order that may induce channeling contrast. Using these new standards, we characterized the mineral content of a broad range of mineralized tissues from immature mouse femur to whale bulla. Mineral volume fraction correlated (p
, Geiss, R.
, Feller, S.
and Ferguson, V.
Tunable glass reference materials for quantitative backscattered electron imaging of mineralized tissues, Journal of Materials Research, [online], https://doi.org/10.1557/jmr.2012.266
(Accessed December 4, 2023)