Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Tunable glass reference materials for quantitative backscattered electron imaging of mineralized tissues

Published

Author(s)

Sara E. Campbell, Roy H. Geiss, Steve A. Feller, Virginia L. Ferguson

Abstract

Backscatter electron (BSE) microscopy provides graylevel contrast resulting from variations in atomic composition. Through the use of standards, quantitative BSE imaging can be used to measure the mineral content of mineralized tissues such as bone and teeth. We developed novel tunable standards that can be adjusted for analysis of an individual tissue or a wide range of tissues with variable mineral content. As an alternative to metallic standards, these amorphous standards maintain long-term stability and possess no long-range order that may induce channeling contrast. Using these new standards, we characterized the mineral content of a broad range of mineralized tissues from immature mouse femur to whale bulla. Mineral volume fraction correlated (p
Citation
Journal of Materials Research
Volume
27
Issue
19

Keywords

scanning electron microscopy, nanoindentation, bone

Citation

Campbell, S. , Geiss, R. , Feller, S. and Ferguson, V. (2012), Tunable glass reference materials for quantitative backscattered electron imaging of mineralized tissues, Journal of Materials Research, [online], https://doi.org/10.1557/jmr.2012.266 (Accessed June 12, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created August 21, 2012, Updated November 10, 2018