In this paper, a novel apparatus and fluorescence detection method for measuring the permeation of H2O2 through polymer barriers (i.e., plastic bottles, caps, and containers) is presented. Measurements were performed with 35 and 50 % (mass/mass) H2O2. Several commercially available food and beverage containers of various thicknesses made from polyethylene terephthalate (PET), both high and low density polyethylene (HDPE and LDPE), polylactic acid (PLA), and polypropylene (PP) were examined. The permeation of H2O2 as a function of time (that is, days in the permeation cell) was determined. Additionally, the permeability of H2O2 across a PET bottle cap was examined.