A major concern while conducting in vitro testing of volatile chemicals, such as halons or their alternatives, is loss of the test chemical during the course of experimental studies. Imprecision in dosimetry confounds experimental results and prevents appropriate comparisons among various chemicals and exposure concentrations. We designed a novel system for exposing cell cultures to volatiles and applied this technology to the toxicity evaluation of 20 halogenated aliphatics. To rank these chemicals, we then derived a composite toxicity index based on six separate in vitro toxicity assays. Our results show the ability to rank a group of chemicals, based on an in vitro toxicity index and the correlation of these results with published toxicity values. This provides a means of discerning relative toxic potencies among groups of chemicals exposed under controlled dosimetry conditions and is especially useful for ranking volatile chemicals in materials development programs.
Citation: Special Publication (NIST SP) - 984-4Report Number:
NIST Pub Series: Special Publication (NIST SP)
Pub Type: NIST Pubs