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Search Publications by: Peter M. Vallone (Fed)

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Displaying 176 - 186 of 186

Quality Control of PCR Primers Used in Multiplex STR Amplifcation Reactions

June 1, 2001
Author(s)
John M. Butler, J E. Devaney, M A. Marino, Peter M. Vallone
Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification

Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions

February 1, 2001
Author(s)
John M. Butler, C M. Ruitberg, Peter M. Vallone
Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining

DNA Mass Spectrometry at NIST

February 1, 2001
Author(s)
Peter M. Vallone
The use of mass spectrometry as a tool for solving biochemical problems is growing. Specifically, matrix assisted laser desorption ionization coupled with time-of-flight detection systems (MALDI-TOF) is emerging for high throughput and highaccuracy

Melting Analysis of DNA Dumbbells with Repeating Dinucleotide Stem Sequences Reveals Evidence for Long Range Sequence Dependent Interactions in Short Duplex DNA

February 1, 2000
Author(s)
Peter M. Vallone, R. Owczarzy, P. V. Riccelli, A. S. Benight
Melting Analysis of DNA Dumbbells with Repeating Dinucleotide Stem Sequences Reveals Evidence for Long Range Sequence Dependent Interactions in Short Duplex DNA. Optical and DSC melting studies were performed on 12 DNAdumbbells in 85 mM Na+. The stems of
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