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Displaying 51 - 75 of 83

Enhancing molecular flux through nanopores via attractive interactions

November 1, 2006
Author(s)
John J. Kasianowicz, Tam Nguyen, Vincent M. Stanford
The classic experiments by Galvani and his colleagues in the 1790s led him to suggest that neural conduction and muscle contraction are governed by a form of animal electricity. Nearly 100 years later, Santiago Ramon y Cajal?s use of Golgi stains provided

Enzyme Activity to Augment the Characterization of Tethered Bilayer Membranes

June 1, 2006
Author(s)
Gintaras Valincius, Duncan J. McGillivray, W. Febo-Ayala, David J. Vanderah, John J. Kasianowicz, Mathias Losche
The structural order of tethered lipid bilayers (tBLMs) on solid substrates, investigated with electrochemical impedance spectroscopy (EIS) and neutron reflectivity (NR), is further characterized by phospholipase activity. Whereas EIS and NR data indicate

Purified Bacillus Anthracis Lethal Toxin Complex Formed In Vitro and During Infection Exhibits Functional and Biological Activity

March 18, 2005
Author(s)
Rekha Panchal, Kelly Halverson, Wilson Ribot, Douglas Lane, Tara Kenny, Teresa G. Abshire, John W. Ezzell, Timothy A. Hoover, Bradford Powell, Stephen Little, John J. Kasianowicz, Sina Bavari
Anthrax protective antigen (PA, 83 kDa), a pore-forming protein upon protease activation to 63 kDa (PA63), translocates lethal factor (LF) and edema factor (EF) from endosomes into the cytosol of the cell. The relatively small size of the heptameric PA63

Micro-Electroplating Silver on Sharp Edges for Fabrication of Solid-State Nanopores

August 11, 2004
Author(s)
Brian J. Polk, Melanie Bernard, John J. Kasianowicz, Martin Misakian, Michael Gaitan
Electrodeposition of silver was investigated as a fabrication tool for infilling large (103 mm2) vias in silicon substrates. Silver reduction from ammoniacal silver nitrate was studied at electrodes of novel geometry, i.e. the edge of the vias, with

Electrostatic Influence on Ion Transport through the alpha HL Channel

October 1, 2003
Author(s)
M Misakian, John J. Kasianowicz
The current-voltage relationship of a single Staphylococcus aureus α-hemolysin(αHL) channel is nonlinear, rectifying, and depends on the bulk pH and the ionic strength. The data are described qualitatively by a simple one-dimensional Nernst-Planck analysis

Phase Transitions Within the Isolated Polymer Molecule: Coupling of the Polymer Threading a Membrane Transition to the Helix-Random Coil, the Collapse, the Adsorption, and the Equilibrium Polymerization Transitions

September 22, 2003
Author(s)
Edmund A. DiMarzio, John J. Kasianowicz
The polymer threading a membrane transition (PTM), which is a first-order thermodynamic phase transition for an isolated linear polymer in the limit of infinite molecular weight, is coupled to the other 4 phase transitions of the isolated polymer molecule

Using HMMs to Quantify Signals from DNA Driven Through a Nanometer Scale Pore

October 1, 2002
Author(s)
Vincent M. Stanford, John J. Kasianowicz
It was recently shown that individual molecules of single-stranded DNA can be forced through a nanoscale pore by an electric field. We demonstrate signal processing methods to detect and measure sub-states in the DNA-induced current blockades during