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Search Publications by: Kenneth D. Cole (Assoc)

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Displaying 51 - 67 of 67

Electrophoretic Capture of Circular DNA in Gels - A Review

August 1, 2002
Author(s)
B. Akerman, Kenneth D. Cole
Results on electrophoretic capture of circular DNA in porous gels are reviewed. Processes, which cause arrest of circular forms of DNA during electrophoresis, can provide a very efficient separation mechanism for the purification of plasmids and bacterial

Separation of Large Circular DNA by Electrophoresis in Agarose Gels

January 1, 2002
Author(s)
Kenneth D. Cole, C M. Tellez
The electrophoresis of circular DNA, ranging in size from 4.4 kilobase pairs (kbp) to 220 kbp, was studied in agarose gels. Bacterial artificial chromosome (BAC) DNA was used as a source of large supercoiled and open circular (relaxed) forms. the open

Preparative Electrochromatography of Proteins in Various Types of Porous Media

March 1, 2000
Author(s)
C M. Tellez, Kenneth D. Cole
The performance of commercial and enzymatically-modified size-exclusion (SE) gels in electrochromatography was compared for preparative protein separations. Dextran and agarose-based SE gels were subjected to enzymatic digestion under mild conditions. This

Separation of Different Physical Forms of Plasmid DNA Using a Combination of Low Electric Field Strength and Flow in Porous Media: Effect of Different Field Gradients and Porosity of the Media

March 1, 2000
Author(s)
Kenneth D. Cole, C M. Tellez-Rodriguez, R W. Blakesley
The retention of different physical forms of DNA by an electric field in a chromatography system was studied. We were able to effectively separate the supercoiled and the open circular forms of plasmid DNA using this type of electrochromatography system
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