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Displaying 676 - 700 of 1612

Spectral Fluorescence Measurements

July 7, 2014
Author(s)
Paul C. DeRose, Joanne C. Zwinkels, James Leland
This chapter covers the topic of spectral fluorescence measurements. These fluorescence measurements offer significant advantages in terms of sensitivity and selectivity, finding wide use in a range of applications in analytical and color technologies. The

Viscosity Measurements of DNA Solutions with and without Condensing Agents

July 7, 2014
Author(s)
Arno D. Laesecke, Jessica L. Burger
Aqueous DNA solutions at three concentrations were studied as model sputum by viscosity measurements at ambient pressure. Initial measurements at 25 °C, 37.78 °C, and 50 °C indicated a viscosity increase toward 50 °C. This phenomenon was studied by

State of the Art Raman Techniques for Biological Applications

July 1, 2014
Author(s)
Angela R. Hight Walker, Alasdair Rae, Rainer Stosch, Petr Klapetek, Debdulal Roy
Raman spectroscopy is a powerful tool for the elucidation of qualitative and quantitative information from biological systems and has huge potential in areas such as biotechnologies, drug discovery, agro-chemical research and clinical diagnostics. This

Standards in Biothermodynamics

May 15, 2014
Author(s)
Robert N. Goldberg
The field of biothermodynamics encompasses physical property measurements on biochemical and biological systems. This chapter reviews the status of standards documents that are pertinent to biothermodynamics as well as recommendations that have been made

Machine Learning Analysis for Identification of Cell Shape Metrics Associated with Stem Cell Differentiation in Nanofiber Scaffolds

April 19, 2014
Author(s)
Desu Chen, Sumona Sarkar, Stephen J. Florczyk, Subhadip Bodhak, Carl G. Simon Jr., Joy P. Dunkers, Julian Candia, Wolfgang Losert
Cell shape has been demonstrated to be closely related to cell function and may be an important predictor of cell fate. Many metrics are available to describe cell shape, however the relationship of these metrics to cell fate are not well understood

Measuring Organelle Shape in 3D in Stem Cells Cultured on Nanofiber Scaffolds

April 19, 2014
Author(s)
Carl G. Simon Jr., Peter Bajcsy, Wojtek J. Tutak, Jyotsnendu J. Giri
Previous work has demonstrated that culture of osteoprogenitor cells on nanofiber scaffolds can potentiate osteogenic differentiation [1-4]. Culture of cells in nanofiber scaffolds causes changes to cell morphology, suggesting that morphological changes

Structural Basis for Inactivation of Giardia lamblia Carbamate Kinase by Disulfiram

April 11, 2014
Author(s)
Andrey Galkin, Liudmila Kulakova, Kap Lim, Catherine Chen, Wei Zhang, Illarion Turko, Osnat Herzberg
Carbamate kinase from Giardia lamblia is an essential enzyme for the survival of the organism. The enzyme catalyzes the final step in the arginine dihydrolase pathway converting ADP and carbamoyl phosphate to ATP and carbamate. We previously reported that

AN EXAMINATION OF METAL DEPOSITION DERIVED FROM AEROSPACE-RELATED ACTIVITY IN SEVERAL ENVIRONMENTAL MATRICES COLLECTED FROM WATERS SURROUNDING THE KENNEDY SPACE CENTER (KSC), FLORIDA

April 8, 2014
Author(s)
John Bowden, Theresa M. Cantu, Douglas M. Scheidt, Russell H. Lowers, Brian A. Nocito, Vaneica Y. Young, Louis J. Guillette
Metal deposition originating from anthropogenic activity was examined in a two-tier study using environmental samples collected from several locations at the Kennedy Space Center (KSC) and surrounding Merritt Island National Wildlife Refuge (MINWR) with

Measuring Stem Cell Dimensionality in Tissue Scaffolds

April 1, 2014
Author(s)
Carl G. Simon Jr., Sapun Parekh, Christopher K. Tison, Girish Kumar, Tanya M. Farooque, Charles H. Camp
Many 3D scaffold systems have evolved for tissue engineering, drug screening and in vitro tissue models. However, it is not clear which scaffolds provide a 3D cell niche and there is no clear way to measure cell niche dimensionality. Advances in 3D niche

Application of Falling-Needle Rheometry to Highly Concentrated DNA Solutions.

March 18, 2014
Author(s)
Jessica L. Burger, Hideki Yamamoto, Takamasa Suzuki, Arno D. Laesecke
High concentration DNA solutions are common both in vitro and in vivo, and understanding the rheological properties is a critical area of bioscience. Our previous measurements on high concentration DNA solutions (2-6 mg/ml) interestingly provided evidence
Displaying 676 - 700 of 1612
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