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Displaying 51 - 75 of 232

Forensic DNA Advisory Groups: DAB, SWGDAM, ENFSI, and BSAG

December 28, 2012
John M. Butler
A number of organizations exist around the world that work on a national or international level to aid quality assurance and to promote accurate forensic DNA testing. These organizations are made up primarily of working scientists who coordinate their

Developmental Validation of the PowerPlex(R) ESI 17 Pro System

December 27, 2012
Carolyn R. Steffen, Margaret C. Kline, John Butler, Robert S. McLaren, Jaynish Patel, Margaret Ewing, Douglas R. Storts, Fabrice Noel, Sophie Dognaux
The SE33 locus is one of the most polymorphic markers used in human identification. However, it also possesses multiple microvariants both within the repeat and in the flanking regions. Such flanking region mutations can generate discordant allele calls

The Effects of Diffusion on an Exonuclease/Nanopore-Based DNA Sequencing Engine

December 7, 2012
Joseph E. Reiner, Joseph W. Robertson, Arvind Balijepalli, Daniel L. Burden, Bryon S. Drown, John J. Kasianowicz
The ability to electronically detect and characterize individual polynucleotides as they are driven through a single protein ion channel may eventually prove useful for rapidly sequencing DNA (base-by-base) in a ticker tape-like fashion. More recently, a

PEG-Labeled Nucleotides and Nanopore Detection for Single Molecule DNA Sequencing by Synthesis

September 21, 2012
Shiv Kumar, Tao Chuanjuan, Chien Minchen, Hellner Brittney, Joseph W. Robertson, Arvind Balijepalli, Li Zengmin, James J. Russo, Joseph E. Reiner, John J. Kasianowicz, Jingyue Ju
There is a significant need to accurately sequence single DNA and RNA molecules for personalized medicine. We describe a novel nanopore-based sequencing by synthesis (SBS) strategy that will accurately differentiate at single molecule level four different

Structural and biochemical studies of a plant formamidopyrimidine-DNA glycosylase reveal why eukaryotic Fpg glycosylases do not excise 8-oxoguanine.

July 11, 2012
M Miral Dizdar, Pawel Jaruga, Susan Duclos, Pierre Aller, Susan Wallace, Susan Doublie
Formamidopyrimidine-DNA glycosylase (Fpg; MutM) is a DNA repair enzyme widely distributed in bacteria. Fpg recognizes and excises oxidatively modified purines, 4,6-diamino-5-formamidopyrimidine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-oxoguanine

Identification and quantification of DNA repair proteins by liquid chromatography/isotope-dilution tandem mass spectrometry using 15N-labeled whole proteins as internal standards

May 30, 2012
M Miral Dizdar, Prasad T. Reddy, Guldal Kirkali, Gamze Tuna, Bryant C. Nelson, Pawel Jaruga
Oxidatively induced DNA damage is implicated in disease, unless it is repaired by DNA repair. DNA repair proteins may be used as early detection and therapeutic biomarkers in cancer and other diseases. For this purpose, the measurement of the expression

Mechanisms of free radical-induced damage to DNA

April 1, 2012
M Miral Dizdar, Pawel Jaruga
Endogenous and exogenous sources cause free radical-induced DNA damage in living organisms by a variety of mechanisms. The highly reactive hydroxyl radical reacts with the heterocyclic DNA bases and the sugar moiety near or at diffusion-controlled rates by

DNA Molecules Descending a Nanofluidic Staircase by Entropophoresis

January 26, 2012
Samuel M. Stavis, Jon C. Geist, Michael Gaitan, Laurie E. Locascio, Elizabeth A. Strychalski
A complex entropy gradient for confined DNA molecules was engineered for the first time. Following the second law of thermodynamics, this enabled the directed self-transport and self-concentration of DNA molecules. This new nanofluidic method is termed

Oxidatively Induced DNA Damage and Cancer

December 30, 2011
M Miral Dizdar, Pawel Jaruga
Oxidatively induced DNA damage is caused by endogenous and exogenous sources in living organisms. Many resulting DNA lesions are mutagenic and lead to mutations commonly found in cancer. Repairs mechanisms exist to repair this type of DNA damage

Evidence for upregulated repair of oxidatively induced DNA damage in human colorectal cancer

September 15, 2011
M Miral Dizdar, Pawel Jaruga, Prasad T. Reddy, Guldal Kirkali, Didem Keles, Gulgun Oktay, Aras E. Canda
Molecular pathways that play a role in the development of colorectal cancer involve multiple genetic changes in cancer-related genes that may be caused by overproduction of oxygen-derived species including free radicals, which are capable of damaging DNA

Stable isotope-labeling of DNA repair proteins, and their purification and characterization

July 1, 2011
M Miral Dizdar, Pawel Jaruga, Prasad T. Reddy, Bryant C. Nelson, Mark S. Lowenthal
Reduced DNA repair capacity is associated with increased risk for a variety of disease processes including carcinogenesis. Thus, DNA repair proteins have the potential to be used as important predictive, prognostic and therapeutic biomarkers in cancer and

PDH-locked, frequency-stabilized cavity ring-down spectrometer

June 16, 2011
Joseph T. Hodges, A. Cygan, Piotr Maslowski, Katarzyna E. Bielska, S. Wojtewicz, J. Domyslawska, Hisashi Abe, R.S. Trawinski, R. Ciurylo
We describe a high sensitivity and high spectral resolution laser absorption spectrometer based upon the frequency-stabilized cavity ring-down spectroscopy (FS-CRDS) technique. We used the Pound-Drever-Hall (PDH) method to lock the probe laser to the high

Metrology Needs and NIST Resources for the Forensic DNA Community

June 1, 2011
Michael D. Coble, Margaret C. Kline, John M. Butler
With the advent of Forensic DNA profiling in the mid-1980s, this technology has had a positive impact on the criminal justice system, helping to convict the guilty and exonerate the innocent. The field has evolved from focusing on multi-locus markers

A Major Role for Non-Enzymatic Antioxidant Processes in the Radioresistance of Halobacterium salinarum

April 1, 2011
M Miral Dizdar, Pawel Jaruga, Courtney K. Robinson, Kim Webb, Amardeep Kaur, Nitin S. Baliga, Allen Place, Jocelyne DiRuggiero
Oxidative stress occurs when the generation of reactive oxygen species (ROS) exceeds the capacity of the cell’s endogenous systems to neutralize them. We analyzed the oxidative stress responses and cellular damage of the archaeon Halobacterium salinarum

Evaluating self-declared ancestry of U.S. Americans using autosomal, Y-chromosomal and mitochondrial DNA

December 31, 2010
Peter Vallone, Oscar Leo, Manfred Kayser, Mannis van Oven, Michael Coble, Toni M. Diegoli, Kristiaan J. van der Gaag, Peter D. Knijff
The current U.S. population represents an amalgam of individuals from different biogeographic ancestries who arrived on the territory at different times in the history of the territory. However, such admixture is far from being homogeneous and this fact

Mechanisms and Measurement of Nanomaterial-Induced Oxidative Damage to DNA

June 22, 2010
Bryant C. Nelson, Elijah J. Petersen
Many of the current investigations on the environmental health and human safety risks of engineered nanomaterials focus on their short-term acute effects. However, the long-term chronic effects of nanomaterials on living systems, and in particular, on the

Development and Demonstration of a Method to Evaluate Bio-Sampling Strategies Using Building Simulation and Sample Planning Software

June 10, 2010
William S. Dols, Jayne B. Morrow, Andrew K. Persily, Brett Matzke, Landon Sego, Lisa Nuffer, Brent Pulsipher
response and recovery sampling approaches and technologies, the U.S. Department of Homeland Security (DHS), along with several other agencies, have simulated a biothreat agent release within a facility at Idaho National Laboratory (INL) on two separate
Displaying 51 - 75 of 232