Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

DNA damage products (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines as potential biomarkers for atherosclerosis in human urine



M Miral Dizdar, Pawel Jaruga, R Olinski, Rafal Rozalski


Rationale: We hypothesized that oxidatively induced DNA damage products (5'R)-8,5'-cyclo-2'-deoxyadenosine (R-cdA) and (5'S)-8,5'-cyclo-2'-deoxyadenosine (S-cdA) may be well-suited as biomarkers for atherosclerosis in human urine. Objective: We tested this hypothesis by accurately measuring the levels of R-cdA and S-cdA in urines of atherosclerosis and healthy individuals. Methods and Results: We collected urine samples from 22 patients with clinical atherosclerosis and from 22 healthy individuals. The levels of R-cdA and S-cdA, and another typical product of DNA damage 8-hydroxy-2'-deoxyguanosine (8-OH-dG) were simultaneously measured using liquid chromatography-tandem mass spectrometry with isotope dilution. We show the presence of R-cdA and S-cdA at significantly greater concentrations in urine of atherosclerosis patients than in that of healthy individuals with a p-value
ACS Biochemistry


atherosclerosis, biomarkers, 8, 5'-cyclo-2'-deoxyadenosines, DNA damage, oxidative stress


, M. , Jaruga, P. , Olinski, R. and , R. (2012), DNA damage products (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines as potential biomarkers for atherosclerosis in human urine, ACS Biochemistry, [online], (Accessed April 23, 2024)
Created February 23, 2012, Updated November 10, 2018