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Bioscience / Engineering/synthetic biology

Genome engineering

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Displaying 1 - 3 of 3

Whole Genome Transplantation: Methodology and Protocol

March 17, 2026

Author(s)

Zumra Seidel, Feilun Wu, Nacrya Assad-Garcia, Carole Lartigue-Pratt, Elizabeth Strychalski, Eugenia Romantseva, Sage Glass, Tyler Goshia, John Glass

Methods to use flow cytometry and sanger sequencing to evaluate CRISPR/Cas9 CD19 knockout in GM24385 cells

June 15, 2022

Author(s)

Sarah Inwood, Linhua Tian, Kirsten Parratt, Samantha Maragh, Lili Wang

CRISPR/Cas9, one of the most commonly used genome editing systems, has been used for production of protein, cell and gene therapies. While an exciting tool, there is not a lot of long-term data about its genomic and phenotypic stability and off-target

Single-cell measurement of plasmid copy number and promoter activity

March 5, 2021

Author(s)

Bin Shao, Jayan Rammohan, Daniel Anderson, Nina Alperovich, David Ross, Christopher Voigt

We have developed a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of

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