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Bioscience / Engineering / synthetic biology

Genome engineering

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Methods to use flow cytometry and sanger sequencing to evaluate CRISPR/Cas9 CD19 knockout in GM24385 cells

June 15, 2022

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Sarah Inwood, Linhua Tian, Kirsten Parratt, Samantha Maragh, Lili Wang

CRISPR/Cas9, one of the most commonly used genome editing systems, has been used for production of protein, cell and gene therapies. While an exciting tool, there is not a lot of long-term data about its genomic and phenotypic stability and off-target

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