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Manufacturing

Biomanufacturing

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Displaying 176 - 200 of 210

Pairwise Alignment of Chromatograms using a Fisher-Rao Metric

September 2, 2014
Author(s)
William E. Wallace, Anuj Srivastava, Kelly H. Telu, Yamil Simon
A new approach to aligning chromatograms is introduced and applied to examples of metabolite identification by liquid chromatography mass spectrometry (LC MS). A square root representation of the chromatogram’s derivative coupled with a Fisher Rao

Trends in QconCATs for targeted proteomics

May 1, 2014
Author(s)
Junjun J. Chen, Illarion Turko
Targeted proteomics has received much attention because of the highly-sensitive, quantitative detection of proteins and post-translational modifications (PTMs). Quantification by targeted proteomics relies on mass spectrometry and isotope-labeled internal

Certification of Total Arsenic in Blood and Urine Standard Reference Materials by Radiochemical Neutron Activation Analysis and Inductively Coupled Plasma Mass Spectrometry

March 1, 2014
Author(s)
Rick L. Paul, William C. Davis, Karen E. Murphy, Colleen E. Bryan Sallee, Lee L. Yu, William F. Guthrie, Dennis D. Leber, Thomas W. Vetter
A newly developed procedure for determination of arsenic by radiochemical neutron activation analysis was used to measure arsenic in SRM 955c Toxic Elements in Caprine Blood and SRM 2668 Toxic Elements in Frozen Human Urine for the purpose of providing

Metabolite Profiling of a NIST Standard Reference Material for Human Plasma (SRM 1950) GC/MS, LC/MS, NMR and Clinical Laboratory Analyses, Libraries and Web-based resources

October 22, 2013
Author(s)
Yamil Simon, Mark S. Lowenthal, Lisa E. Kilpatrick, Maureen L. Sampson, Kelly H. Telu, Paul A. Rudnick, William G. Mallard, Daniel W. Bearden, Tracey B. Schock, Dmitrii V. Tchekhovskoi, Niksa Blonder, Xinjian Yan, Yuxue Liang, Yufang Zheng, William E. Wallace, Pedatsur Neta, Karen W. Phinney, Alan T. Remaley, Stephen E. Stein
Recent progress in metabolomics and the development of increasingly sensitive analytical techniques have renewed interest in global profiling, i.e., semi-quantitative monitoring of all chemical constituents of biological fluids. In this work, we have

Effects of Desialylation on Human alpha1-Acid Glycoprotein-Ligand Interactions

September 16, 2013
Author(s)
Richard Y. Huang, Jeffrey W. Hudgens
Human α1-Acid Glycoprotein (AGP), an acute phase glycoprotein, exists predominantly in blood. With its ability to bind basic, lipophilic, and acidic drugs, AGP has served as a drug carrier. It has been shown that the carbohydrate composition of AGP changes

Segmented chirped-pulse Fourier transform submillimeter spectroscopy for broadband gas analysis

August 15, 2013
Author(s)
Justin L. Neill, Brent J. Harris, Amanda L. Steber, Kevin O. Douglass, David F. Plusquellic, Brooks H. Pate
Chirped-pulse Fourier transform spectroscopy has recently been extended to millimeter wave spectroscopy as a technique for the characterization of room-temperature gas samples. Here we present a variation of this technique that significantly reduces the

The Metrology of Counting Protein Particles

July 13, 2013
Author(s)
Dean C. Ripple, Michael J. Carrier, Richard E. Cavicchi, Christopher B. Montgomery, Zhishang Hu
A common degradation pathway for protein-based drugs is the growth of protein aggregates or particles. Counting and characterization of these particles is needed to assure the quality, efficacy, and safety of this type of drug. The unusual physical

The Metrology of Counting Protein Particles

March 18, 2013
Author(s)
Dean C. Ripple, Michael J. Carrier, Richard E. Cavicchi, Christopher B. Montgomery, Zhishang Hu
A common degradation pathway for protein-based drugs is the growth of protein aggregates or particles. Counting and characterization of these particles is needed to assure the quality, efficacy, and safety of this type of drug. The unusual physical

Mouse Cell Line Authentication

February 13, 2013
Author(s)
Jamie L. Almeida, Carolyn R. Steffen, Kenneth D. Cole
The scientific community has responded to the misidentification of human cell lines with validated methods to authenticate these cells; however, there are few assays available for nonhuman cell line identification. We have developed a multiplex polymerase

Breast Cancer Biomarker Measurements and Standards

January 24, 2013
Author(s)
Kenneth D. Cole, Lili Wang, Hua-Jun He
Cancer is a heterogeneous disease characterized by changes in the levels and activities of important cellular proteins, including oncogenes and tumor suppressors. Genetic mutations cause changes in protein activity and protein expression levels that result

Collaborative Robotics: Measuring Blunt Force Impacts on Humans

October 12, 2012
Author(s)
Joseph A. Falco, Jeremy A. Marvel, Richard J. Norcross
Robot manufacturers are developing a new generation of industrial robots that are designed to work in collaborative environments in close proximity to humans. In parallel, an international standards effort is developing a technical specification to support

Protein Particles: What We Know and What We Dont Know

June 26, 2012
Author(s)
Dean C. Ripple, Mariana Dimitrova
All therapeutic protein products contain particles formed by the aggregation of protein monomers. There is a growing interest in the understanding of particles in biopharmaceutical products, which has been fostered on the one hand by significant

A Lectin-Based Gold Nanoparticle Assay for Probing Glycosylation of Glycoproteins

April 17, 2012
Author(s)
Germarie Sanchez-Pomales, Todd A. Morris, James Falabella, Michael J. Tarlov, Rebecca A. Zangmeister
We report a glycoanalysis method in which lectins are used to probe the glycans of glycoproteins that are adsorbed on gold nanoparticles. A model mannose-presenting glycoprotein, ribonuclease B (RNase B), and the therapeutic monoclonal antibody (mAb)

Carbohydrate-Functionalized Surfactant Vesicles for Controlling Glycan Density

March 15, 2012
Author(s)
Monique A. Makos, Rebecca A. Zangmeister
We report on the development of a method for rapidly characterizing the glycan binding properties of lectins. Catanionic surfactant vesicles were prepared that spontaneously formed in water and remained stable at room temperature for months. By varying the

Standards for the Optical Detection of Protein Particulates

January 23, 2012
Author(s)
Dean C. Ripple, Michael J. Carrier, Joshua R. Wayment
Particulates in protein therapeutics, composed of agglomerated protein monomers, may cause an immunogenic response in patients. Consequently, industry and the FDA desire more accurate methods for counting and characterizing particulates. Unlike likely

PDH-locked, frequency-stabilized cavity ring-down spectrometer

June 16, 2011
Author(s)
Joseph T. Hodges, A. Cygan, Piotr Maslowski, Katarzyna E. Bielska, S. Wojtewicz, J. Domyslawska, Hisashi Abe, R.S. Trawinski, R. Ciurylo
We describe a high sensitivity and high spectral resolution laser absorption spectrometer based upon the frequency-stabilized cavity ring-down spectroscopy (FS-CRDS) technique. We used the Pound-Drever-Hall (PDH) method to lock the probe laser to the high
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