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Bioscience

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Displaying 301 - 325 of 2353

Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2

May 3, 2021
Author(s)
Peter Vallone, James Huggett, Vladimir Benes, Jeremy Garson, Kathryn Harris, Mikael Kubista, Timothy McHugh, Jacob Moran-Gilad, Tania Nolan, Michael Pfaffl, Marc Salit, Greg Shipley, Jo Vandesompele
Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross

Filling the Pharmacopeial Gaps of Visual Inspection Toward Standardization and Consistency of Visible Particle Testing

May 3, 2021
Author(s)
Linda Narhi, George M. Bou-Assaf, Kristen Gonzalez, Maryam Mazaheri, Steven Messick, Srivalli Telikepalli
The presence of visible particles that are not anticipated to be present in drug products is one of the most common causes of recalls, and warning letters1, 2. This critical quality attribute, which is typically determined during visual inspection (along

Revision of the SNPforID 34-plex forensic ancestry test: Assay enhancements, standard reference sample genotypes and extended population studies

May 3, 2021
Author(s)
John Butler, Manuel Fondevila, Carla Santos, Ana Freire, Christopher Phillips, M Lareu, Peter Vallone
A revision of an established 34 SNP forensic ancestry test has been made by swapping the under- performing rs727811 component SNP with the highly informative rs3827760 that shows a near-fixed East Asian specific allele. We collated SNP variability data for

The San Francisco Estuary Institute Collection at the NIST Biorepository

April 28, 2021
Author(s)
Debra Ellisor, Rebecca Pugh, Nina Buzby, Michael Weaver, Melissa Foley
The National Institute of Standards and Technology (NIST) has been collaborating with the San Francisco Bay Estuary Institute (SFEI) since 2009, providing biobanking services at the NIST Biorepository in Charleston, South Carolina in support of their

Sequence-based U.S. population data for 7 X STR loci

April 26, 2021
Author(s)
Lisa Borsuk, Carolyn R. Steffen, Kevin M. Kiesler, Peter Vallone, Katherine Gettings
The National Institute of Standards and Technology (NIST) U.S. population sample set of unrelated individuals was used to determine allele and haplotype frequencies for seven X-chromosome short tandem repeat (STR) loci in four linkage groups. DXS7132

The NIH Somatic Cell Genome Editing program

April 7, 2021
Author(s)
Samantha Maragh
The move from reading to writing the human genome offers new opportunities to improve human health. The United States National Institutes of Health (NIH) Somatic Cell Genome Editing (SCGE) Consortium aims to accelerate the development of safer and more

ISCMA: An Information Security Continuous Monitoring Program Assessment

March 31, 2021
Author(s)
Victoria Yan Pillitteri, Kelley L. Dempsey, Chad Baer, Ron Rudman, Robert Niemeyer, Susan Urban
This publication describes an example methodology for assessing an organization's Information Security Continuous Monitoring (ISCM) program. It was developed directly from NIST guidance and is applicable to any organization, public or private. It can be

Biobanking in the COVID-19 Era and Beyond: Part 1. How Early Experiences can Translate into Actionable Wisdom

March 26, 2021
Author(s)
Clare M. Allocca, Marianna J. Bledsoe, Monique Albert, Sergey Anisimov, Elena Bravo, Marta Castelhano, Yehudit Cohen, Mieke DeWilde, Koh Furuta, Zisis Kozlakidis, Dunja Martin, Anabela Martins, Helen Morrin, Shannon McCall, Rebecca Pugh
The era of COVID-19 has brought about a number of novel challenges for the global biobanking community. To better position the biobanking community to cope with current and future challenges, the International Society for Biological and Environmental

Biobanking in the COVID-19 Era and Beyond: Part 2. A Set of Tool Implementation Case Studies

March 26, 2021
Author(s)
Clare M. Allocca, Emma Snapes, Monique Albert, Marianna J. Bledsoe, Marta Castelhano, Mieke DeWilde, Koh Furuta, Zisis Kozlakidis, Dunja Martin, Anabela Martins, Shannon McCall, Brent Schacter
The COVID-19 era has brought about a number of novel challenges for the global biobanking community. An array of diverse tools exists to support quality and fitness-for-purpose in biobank operations. The International Society for Biological and

CD19 Expression Analysis Using Two Flow cytometric Quantitative Schemes: An approach for Identifying Robust B-cell Reference Control Materials

March 19, 2021
Author(s)
Hao-Wei Wang, Paul Patrone, Anthony Kearsley, Jessica Watson, Liang Lim, Jothir Pichaandi, Olga Ornatsky, Daniel Majonis, Rukmini Bhardwaj, Howard Mostowski, Steven Bauer, Heba Degheidy, Lili Wang
Cell-based therapies have emerged as a novel approach to treat cancer and other conditions. Quantitative flow cytometry plays an increasingly important role in predicting therapy outcome in a wide range of hematologic malignancies. Several studies

Measurement of serological and neutralizing antibodies specific to SARS-CoV-2 virus in humans with COVID-19 disease

March 8, 2021
Author(s)
Linhua Tian, Elzafir B. Elsheikh, Paul Patrone, Anthony Kearsley, adolfas Gaigalas, Sarah L. Inwood, Sheng Lin-Gibson, Dominic Esposito, Lili Wang
The global response to COVID-19 has led to rapid advances in diagnostic, surveillance, and vaccine development. Critical measurements underpinning many of these efforts include serological assays to assess seroprevalence of populations and the complex

Single-cell measurement of plasmid copy number and promoter activity

March 5, 2021
Author(s)
Bin Shao, Jayan Rammohan, Daniel Anderson, Nina Alperovich, David J. Ross, Christopher Voigt
We have developed a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of

Report of the 2019 NIST-FDA Workshop on Standards for Next Generation Sequencing Detection of Viral Adventitious Agents in Biologics and Biomanufacturing

February 22, 2021
Author(s)
Megan Cleveland, Bharathi Anekella, Mike Brewer, Pei-Ju Chin, Heather Couch, Eric Delwart, Jim Huggett, Scott Jackson, Javier Martin, Serge Monpoeho, Tom Morrison, Siemon Ng, David Ussery, Arifa Khan
Adventitious virus testing assures product safety by demonstrating the absence of viruses that may have been unintentionally introduced during the manufacturing process. The capabilities of next-generation or high-throughput sequencing (NGS/HTS) for broad

Conformational Changes of Immobilized Polythymine due to External Stressors Studied with Temperature-Controlled Electrochemical Microdevices

February 17, 2021
Author(s)
Ramya Vishnubhotla, Sarah Robinson, Jon R. Askim, Christopher B. Montgomery, Kristen L. Steffens, Herman O. Sintim, Stephen Semancik
Conformational changes of single-stranded DNA (ssDNA) play an important role in a DNA strand's ability to bind to target ligands. A variety of factors can influence conformation, including temperature, ionic strength, pH, buffer cation valency, strand
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