NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Bioscience

Search Publications

Search Title, Abstract, Conference, Citation, Keyword or Author
  • Published Date
Displaying 1 - 25 of 1242

NIST 2025 Rapid Microbial Testing Methods Workshop Report

September 26, 2025
Author(s)
Jason Kralj, Kirsten Parratt, Tyler Laird, Stephanie Servetas, Nancy Lin, Dawn Henke, Scott Jackson
In 2020, the National Institute of Standards and Technology (NIST)-led Rapid Microbial Testing Methods (RMTM) Consortium was established to develop standards and measurement-based solutions to facilitate the adoption of RMTMs in advanced therapy products

Report from the 2023 NIST-Hosted Workshop on Collaborative Efforts to Enable Adoption of Rapid Microbial Testing Methods for Advanced Therapy Products

September 26, 2025
Author(s)
Stephanie Servetas, Dawn Henke, Jason Kralj, Kirsten Parratt, Scott Jackson, Nancy Lin
On April 25, 2023, the National Institute of Standards and Technology (NIST) hosted a one-day hybrid workshop focused on bringing together organizations and working groups with the common goal of enabling validation and implementation of rapid microbial

NIST 2024 Rapid Microbial Testing Methods Workshop Report

September 25, 2025
Author(s)
Jason Kralj, Nancy Lin, Kirsten Parratt, Stephanie Servetas, Dawn Henke, Scott Jackson
The NIST-led Rapid Microbial Testing Methods (RMTM) Consortium launched in 2020 to develop standards and measurement-based solutions to advance the use of RMTMs in advanced therapy products. NIST hosted 4th annual workshop on RMTMs to update the community

Securing the Future of NMR Metabolomics Reproducibility: A Call for Standardized Reporting

September 15, 2025
Author(s)
Tracey Johnston, Fabio Casu, Amanda Bayless, Erik Andersson, Robert Brua, Munki Choo, Arthur S. Edison, Hamid R. Eghbalnia, Candace Fleischer, Goncalo J Gouveia, Jeffrey C. Hoch, Gagandeep Kaur, Da-Wei Li, Wimal Pathmasiri, Istvan Pelczer, Fay Probert, Daniel Raftery, David Rovnyak, Michael Secreto, Panteleimon Takis, Mario Uchimiya, David S Wishart, Ali Yilmaz, Lloyd Sumner, Robert Powers, Valerie Copie, Teklab Gebregiworgis
Metabolomics has been a rapidly growing multidisciplinary field with ever increasing demand and usability, attracting a surge of new researchers. While their varied skill sets, questions, and approaches enrich the field with fresh perspectives and

Topology and Kinetic Pathways of Colloidosome Assembly and Disassembly

September 4, 2025
Author(s)
Raymond Adkins, Joanna Robaszewski, Seungwoo Shin, Fridtjof Brauns, Leroy Jia, Ayantika Khanra, Prerna Sharma, Robert Pelcovits, Thomas Powers, Zvonimir Dogic
Liquid shells, such as lipid vesicles, emulsions, and soap bubbles, are ubiquitous throughout biology, engineered matter, and everyday life. Their creation or disintegration is defined by a singularity separating topologically distinct one-boundary

Characterizing Cell-Free Transcription and Translation Dynamics with Nucleic Acid-Based Assays

August 10, 2025
Author(s)
Fernanda Piorino Macruz de Oliveira, Chad Alan Sundberg, Elizabeth Strychalski, Eugenia Romantseva
Characterization of cell-free expression (CFE) systems must expand beyond single spectrophotometric measurements of a green fluorescent protein to provide meaningful metrics of system performance over the course of a CFE reaction and enable the development

Copper acquisition in Bacillus subtilis involves Cu(II) exchange between YcnI and YcnJ

July 14, 2025
Author(s)
Yuri Rafael de Oliveira Silva, Grayson Barnes, Dia Zheng, Samuel Geathers, Stephen C. Peters, Veronika Szalai, John D. Helmann, Oriana S. Fisher
The transition metal copper is biologically essential across all three domains of life. Several copper-dependent proteins and enzymes produced by the Gram-positive bacterium Bacillus subtilis have been characterized. However, many questions remain about

Inter-tool analysis of a NIST dataset for assessing baseline nucleic acid sequence screening

July 10, 2025
Author(s)
Tyler Laird, Kevin Flyangolts, Craig Bartling, Bryan Gemler, Jacob Beal, Tom Mitchell, Steven T. Murphy, Jens Berlips, Leonard Foner, Ryan Doughty, Felix Qunitana, Michael Nute, Todd J. Treangen, Gene D. Godbold, Krista Ternus, Tessa Alexanian, Nicole Wheeler, Samuel Forry
Nucleic acid synthesis is a dual-use technology that can benefit fields such as biology, medicine, and information storage. However, synthetic nucleic acids could also potentially be used negligently and ultimately cause harm, or be used with malicious

Genomic infrastructure for cetacean research and conservation: Reference genomes for eight families spanning the cetacean phylogeny

July 2, 2025
Author(s)
Phillip A Morin, Bernhard Bein, Chiara Bortoluzzi, Yury V Bukhman, Taylor Hains, Dorothea Heimeier, Marcela Uliano-Silva, Dominic Absalon, Linelle Abueg, Jessica Antosiewicz-Bourget, Robin W Baird, Jennifer R Balacco, Robert K Bonde, Nadolina Brajuka, Andrew Brownlow, Emma Carroll, Molly Carter, Joanna Collins, Nick Davison, Amy Denton, Olga Dudchenko, Olivier Fedrigo, Giulio Formenti, Andy Foote, Guido Gallo, Carola Greve, Marlys Houck, Caroline Howard, Jeff K Jacobsen, Nivesh Jain, Ksenia Krasheninnikova, Erez Lieberman-Aiden, Brigid Maloney, Bethan Manley, Tom Mathers, Shane McCarthy, Michael McGowen, Susanne Meyer, Jacquelyn Mountcastle, Benjamin Neely, Brian O'Toole, Sarah Pelan, Patricia E Rosel, Teri K Rowles, Oliver A Ryder, Tillman Schell, Ying Sims, Judy St. Leger, Ron Stewart, Kerstin Ternes, James A Thomson, Tatiana Tilley, Conor Whelan, Jo Wood, Michael Hiller, Mark Blaxter, Erich D Jarvis
Reference genomes from representative species across families provide the critical infrastructure for research and conservation. The Cetacean Genomes Project (CGP) began in early 2020 to facilitate the generation of near error-free, chromosome-resolved

Proteomics approaches to ecoimmunology: new insights into wildlife immunity and disease

June 24, 2025
Author(s)
Amanda Vicente-Santos1, Natalia Herrera, Gabor Czirjak, Benjamin Neely, Daniel Becker
Understanding wildlife immune responses is crucial for assessing disease risks, environmental stress effects, and conservation challenges. Traditional ecoimmunology approaches rely on targeted assays, which, while informative, often provide a fragmented

VerteBrain reveals novel neuronal and non-neuronal protein assemblies conserved across vertebrate brains

May 28, 2025
Author(s)
Vy Dang, Brittney Voigt, David Yang, Gabriel Hoogerbrugge, Muyoung Lee, Rachael Cox, Ophelia Papoulas, Claire McWhite, Raksha Pradeep, Janelle Leggere, Benjamin Neely, Ryan Gray, Edward Marcotte
Protein-protein interactions in the vertebrate brain underlie key processes such as neurotransmitter release, receptor activation, and signaling pathways that are essential for learning, memory, and cognition. Here, we systematically map protein-protein

Elongated particles in flow: commentary on small-angle scattering investigations

May 23, 2025
Author(s)
Guan-Rong Huang, Lionel Porcar, Ryan Murphy, Yuya Shinohara, Yangyang Wang, Jan-Michael Carrillo, Bobby G. Sumpter, Chi-Huan Tung, Lijie Ding, Changwoo Do, Wei-Ren Chen
This work thoroughly examines several analytical tools, each possessing a different level of mathematical intricacy, for the purpose of characterizing the orientation distribution function of elongated objects under flow. Our investigation places an

Top-Down Scoring of Spectral Fitness by Image Analysis for Protein Structure Validation

May 6, 2025
Author(s)
Benjamin Harding, Barry DeZonia, Rajat Garg, Ziling Hu, Frank Delaglio, Tim Grant, Chad Rienstra
Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique for protein structure determination, but traditional approaches require extensive manual assignment of hundreds to thousands of resonances. Here we present NMRFAM-BPHON, a novel "top

Certification of Standard Reference Material(R) 1989 Monodisperse Irregularly Shaped Epoxy-Based Particles (Nominal 100 mm, 150 mm, 220 mm)

May 2, 2025
Author(s)
Srivalli Telikepalli, Dean Ripple, Michael Carrier, Kristen Steffens, David Newton, Christopher Montgomery, Nicholas Ritchie, Anthony Asmar, Michael Halter
Standard Reference Material (SRM) 1989 is a NIST particle standard produced using photolithographic methods and delivers a certified value for particle size of an irregularly shaped particle. This SRM was developed to allow a more accurate monitoring of

Interlaboratory Assessment of Candidate Reference Materials for Lentiviral Vector Copy Number and Integration Site Measurements

April 21, 2025
Author(s)
Hua-Jun He, Zhiyong He, Steven Lund, Barbara Paugh, Jennifer McDaniel, Justin Zook, Sierra Miller, Samantha Maragh, Simona Patange, Mahir Mohiuddin, Alessandro Tona, John Elliott, Kenneth Cole, Sheng Lin-Gibson
Reference materials are essential for accurately measuring integrated lentiviral vector (LV) copy number (VCN) and integration sites for the safety and efficacy of lentivirus-based gene therapy. We conducted an interlaboratory study on NIST candidate

Recommended Nomenclature Convention for the NISTCHO Cell Line and Its Product Monoclonal Antibody, cNISTmAb.

April 11, 2025
Author(s)
Megan Cleveland, Ioannis Karageorgos, John Marino, Michael Tarlov, Katharina Yandrofski, Rebecca Zangmeister, Zvi Kelman
A Chinese hamster ovary (CHO) cell line expressing the same amino acid sequences of the heavy and light chains of the NIST monoclonal antibody, NISTmAb Reference Material 8671, was generated by MilliporeSigma to be developed by NIST as a reference material

Development and characterization of the NISTCHO reference cell line

March 18, 2025
Author(s)
Hussain Dahodwala, Irfan Hodzic, Alexei Slesarev, Ben Cutak, Alexander Kuzin, Rahul Lal, Jiajian Liu, James Mahon, Rajagopalan Lakshmi Narasimhan, Jaya Onuska, James Ravellette, Kelsey Reger, Sadie Sakurada, Floy Stewart, Trissa Borgschulte, Colette Cote, Kelvin Lee, Zvi Kelman, William Brad O'Dell, Britta Anderson
Well characterized reference materials enable successful collaborations within the scientific community by establishing common reagents for benchmarking studies and reducing the barriers to sharing materials and information. Here, we report the development

Measuring the size of oil droplets in a flow cytometer using Mie Resonances

January 21, 2025
Author(s)
Richard E. Cavicchi, Dean Ripple, Joshua Welsh, Jerilyn Izac, Alexander Peterson, Aaron Goldfain, Wyatt Vreeland
An emulsion of silicone oil droplets in aqueous buffer produces a distinctive series of peaks or resonances in the side scatter histogram in a flow cytometer. As many as 12 peaks are observed in the violet-side scatter channel at 405 nm, with half that

Interlaboratory Measurement of Adeno-Associated Virus: Comparative Quantification of Full and Empty Capsids

December 26, 2024
Author(s)
Sean Lehman, Wyatt Vreeland, Jerome Jacques, Sharee Adams-Hall, Shreya Ahuja, Adnan Arnaout, Hunter Balduf, Ivan Budyak, Thomas Cleveland, Dennis Delgado, James Deng, Brandon Doyle, David Duewer, Carsten Elger, Jeffrey Fagan, Tim Guo, Jorge Haller, Luisa Hilgenfeld, Van Hoang, Allison Huldin, Matthew Hyatt, Sambit Kar, Sandeep Kedia, Bashkim Kokona, Amy Liu, Li Ma, Diane McCarthy, He Meng, Easton Noble, Veronika Oettle, Andrew Pla, Thomas Powers, Dean Ripple, Herbert Runnels, Raphael Ruppert, Florian Semmelmann, Christopher Sims, Saurabh Singh, Ronald Toth, Austin Vogt, Sabine Wenzel, Neal Whitaker, Zhiwen Yang, Brandon Zhuang
Recombinant adeno-associated virus (AAV) is one of the main viral vector-based gene therapy platforms. AAV is a virus consisting of a ≈25 nm diameter capsid with a ≈4.7 kb cargo capacity. The safety and efficacy depend on the correct encapsidation of the
Was this page helpful?