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Revisiting Shape Selectivity in Liquid Chromatography for Polycyclic Aromatic Hydrocarbons (PAHs) - Six-Ring and Seven-Ring Cata-condensed PAH Isomers of Molecular Mass 328 Da and 378 Da

Published

Author(s)

Jorge O. Ona Ruales, Lane C. Sander, Walter Brent Wilson, Stephen Wise

Abstract

The relationship of reversed-phase liquid chromatography retention on a polymeric C18 stationary phase and the shape of polycyclic aromatic hydrocarbons (PAHs) was investigated for three-ring to seven-ring cata-condensed isomers. We report the first reversed-phase LC separation for six-ring and seven-ring cata-condensed PAH isomers. Correlations of LC retention and shape parameters (length-to-breath ratio, L/B and thickness, T) were investigated for 2 three-ring (molecular mass 178), 5 four-ring isomers (molecular mass 228 Da), 11 five-ring isomers (molecular mass 278), 17 six-ring isomers (molecular mass 328 Da), and 20 seven-ring isomers (molecular mass 378 Da). Significant linear correlations were found for all isomer groups (r = 0.71 to 0.94). Nonplanarity of the PAH isomers was found to influence retention (i.e., nonplanar isomers eluting earlier than expected based on L/B) and linear correlations of retention vs. T for isomer groups containing nonplanar isomers were significant (r = 0.71 to 0.86).
Citation
Analytical and Bioanalytical Chemistry

Keywords

Polycyclic Aromatic Hydrocarbons (PAHs), Reverse-Phase Liquid Chromatography (RPLC), Shape Selectivity, Length-to-Breadth Ratio, Nonplanarity, High Molecular Mass PAHs

Citation

Ona Ruales, J. , Sander, L. , Wilson, W. and Wise, S. (2017), Revisiting Shape Selectivity in Liquid Chromatography for Polycyclic Aromatic Hydrocarbons (PAHs) – Six-Ring and Seven-Ring Cata-condensed PAH Isomers of Molecular Mass 328 Da and 378 Da, Analytical and Bioanalytical Chemistry, [online], https://doi.org/10.1007/s00216-017-0456-x (Accessed April 19, 2024)
Created June 27, 2017, Updated November 29, 2022