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Quantification of total serum transferrin and transferrin sialoforms in human serum; an alternative method for the determination of carbohydrate-deficient transferrin in clinical samples

Published

Author(s)

Yoana Y. Nuevo Ordonez, William C. Davis, Raymond F. Anton

Abstract

Carbohydrate deficient transferrin (CDT) measurements in human serum constitute the most specific marker for chronic heavy alcohol use and congenital disorders of glycosylation in proteins. Therefore, the development of precise and accurate analytical methods for individual CDT determination is of significant importance because reliable CDT standards are, so far, not commercially available. We describe an improved HPLC-Sector Field Inductively Coupled Plasma Mass Spectrometry (HPLC-SF-ICP-MS) method for relative and absolute quantification of the different glycoforms in human serum as well as providing data for a well-characterized Standard Reference Material for transferrin to which clinical assays for CDT could be standardized.
Citation
The Royal Society of Chemistry

Keywords

Carbohydrate Deficient Transferrin, ICP-MS, serum, HPLC

Citation

Nuevo, Y. , Davis, W. and Anton, R. (2014), Quantification of total serum transferrin and transferrin sialoforms in human serum; an alternative method for the determination of carbohydrate-deficient transferrin in clinical samples, The Royal Society of Chemistry, [online], https://doi.org/10.1039/c4ay00159a (Accessed April 14, 2024)
Created January 20, 2014, Updated January 27, 2020